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pDONR223_HRAS_p.Q61R

Plasmid

#81311

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

Q61R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT3_WT

Plasmid

#82093

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-B

Plasmid

#200825

Purpose

AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-C

Plasmid

#200826

Purpose

AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-D

Plasmid

#200827

Purpose

AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag AMHR2

Plasmid

#20424

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AMHR2 (AMHR2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

C473G mutation when compared to Genbank reference…

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_IDH2_p.R172M

Plasmid

#81618

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

R172M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.Q79K

Plasmid

#81479

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Mutation

Q79K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.D33E

Plasmid

#81659

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

D33E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKBKG_WT

Plasmid

#82242

Purpose

Gateway Donor vector containing IKBKG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKBKG (IKBKG Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-501*-3xFLAG

Plasmid

#174160

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 501-1208

Promoter

EF1A

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-217*-3xFLAG

Plasmid

#174161

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 217-1208

Promoter

EF1A

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK6_p.D387N

Plasmid

#81712

Purpose

Gateway Donor vector containing MAPK6, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK6 (MAPK6 Human)

Use

Gateway entry vector

Mutation

L290V; D387N; del amino acids 1-8

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_WT

Plasmid

#81938

Purpose

Gateway Donor vector containing HRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_p.L146S

Plasmid

#81606

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

L146S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.Q61L

Plasmid

#81302

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_ERBB2_p.V842I

Plasmid

#81722

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

I655V; V842I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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KRAS-A146T

Plasmid

#154426

Purpose

A146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R658Q

Plasmid

#81502

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Mutation

R658Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132L

Plasmid

#81679

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Mutation

R132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge