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C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

Plasmid

#162955

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

Plasmid

#162954

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E34: attB5r-iRFP670-nostop-attB1r

Plasmid

#162947

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

far-red iRFP670 fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E29: attB5r-mKate2-nostop-attB1r

Plasmid

#162946

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate2 fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E15: attB5r-mVenus-nostop-attB1r

Plasmid

#162943

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r

Plasmid

#162934

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

Plasmid

#162933

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-maltose binding protein fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

Plasmid

#162932

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion with AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E21: attB4-PGKp>-attB1r

Plasmid

#162921

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E07: attB2r-HA-attB3

Plasmid

#162911

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

HA epitope tag

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSC8

Plasmid

#50428

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC7

Plasmid

#50427

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC6

Plasmid

#50426

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC5

Plasmid

#50425

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC4

Plasmid

#50424

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC3

Plasmid

#50423

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC2

Plasmid

#50422

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC10

Plasmid

#50430

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-hTie1-FLAG

Plasmid

#119233

Purpose

Plasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.

Depositor

Kari Alitalo

Insert

TIE1 (TIE1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

P hUbC

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pfa6a PhoCl-TsCC(B)::KANMX

Plasmid

#185906

Purpose

Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

Depositor

Matthew Good

Publication

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

PhoCl-TsCC(B)

Tags

PhoCl fluorophore with C-terminal TsCC(B)

Expression

Yeast

Promoter

SP6

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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