We narrowed to 16,183 results for: nes
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puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
Tags3xFlag-3xHAExpressionInsectAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_103 SpCas9 T1337C
Plasmid#179525PurposeFor bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tagDepositorInsertSpCas9 T1337C
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged threonine 1337 to cysteineAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-HA-mIFITM1-C49,50,83A
Plasmid#58420PurposeExpresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)DepositorInsertIFITM1 (Ifitm1 Mouse)
TagsHAExpressionMammalianMutationCysteines 49, 50, and 83 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM054_pMi[3xP3::mCherry-SV40, ivory1.5kb_prom::LifeAct-mBaoJin-P10UTR]
Plasmid#261231PurposeMinos transgenesis construct for Lepidoptera expressing a LifeAct F-actin label in developing wing scalesDepositorInsert3xP3::mCherry-SV40, ivory1.5kb_prom::LifeAct-mBaoJin-P10UTR
ExpressionInsectPromoter3xP3 ; ivory1.5kb_promAvailable sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
p_sc-eVIP-delta-Puro-EGFP-FES
Plasmid#249454PurposeTransfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systemsDepositorAvailable sinceJune 16, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV_3x_U6_Ctnnb1_CAG_nlsTagBFP2
Plasmid#247933PurposeTargeting Ctnnb1 gene in vivo by adeno-associated viral vector-mediated deliveryDepositorInsertCtnnb1 sgRNAs (Ctnnb1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsnls-TagBFP2ExpressionMammalianPromoterU6Available sinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV_3x_U6_Ikbkg_CAG_nlsTagBFP2
Plasmid#247934PurposeTargeting Ikbkg gene in vivo by adeno-associated viral vector-mediated deliveryDepositorInsertIkbkg sgRNAs (Ikbkg Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsnls-TagBFP2ExpressionMammalianPromoterU6Available sinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Renilla-FLAG-firefly-K529E
Plasmid#255007PurposeUsed to test the rate of amino acid misincorporation in mammalian cellsDepositorInsertRenilla-FLAG-firefly-K529E
UseLuciferaseTags3xFlagExpressionMammalianMutationfirefly-K529EPromoterCMVAvailable sinceMay 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
puDNA5_SSF_DOR_MRS_2Ala_APEX2
Plasmid#250763PurposeMammalian cell expression of the delta opioid receptor with the last 17 amino acids of the mu opioid receptor, in which the leucines in the LENL sequence have been mutated to alaninesDepositorInsertDOR-MRS(2Ala)
TagsAPEX2 and Signal Sequence FLAGExpressionMammalianMutationdelta opioid receptor with last 17 amino acids of…PromoterUBCAvailable sinceApril 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-dCas9-3XmCherry-CRY2
Plasmid#249580PurposeLentiviral vector for stable expression of dCas9-3XmCherry-CRY2hiclu fusion protein in mammalian cellsDepositorInsertsCRY2hiclu
Sp dCas9
UseLentiviralTags3XmCherryExpressionMammalianMutationCatalytically dead mutant Cas9PromoterCMVAvailable sinceMarch 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-His6-TEV-SpyCatcher003-cys
Plasmid#249543PurposeExpresses SpyCatcher003 with a N-terminal 6x-His tag and a C-terminal cysteine in E.coli under the T7 promoterDepositorPublicationInsertSpyCatcher003-cys
Tags6x His and CysteineExpressionBacterialPromoterT7Available sinceMarch 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHS484_10xHis_MBP_TEV_dSpRY
Plasmid#244832PurposeBacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purificationDepositorInsertdSpRY Cas9
UseCRISPRTags10xHis-MBPExpressionBacterialMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterT7Available sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TO-HA-Δ37 PPM1H-mito
Plasmid#208375PurposeLentiviral expression of human Δ37-PPM1H with C-terminal mito signalDepositorAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TO-Δ37 PPM1H -PACT-HA
Plasmid#208373PurposeLentiviral expression of human Δ37-PPM1H with C-terminal HA tagDepositorAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TO-Δ37 PPM1H-TMEM115-HA
Plasmid#208374PurposeLentiviral expression of human Δ37-PPM1H with C-terminal TMEM115-HADepositorAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-AP4-cyto
Plasmid#229694PurposeTransient expression of EGFP AP4-cyto in mammalian cellsDepositorInsertEGFP; Control construct with tandem AP4 motifs (cytoplasm)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-PPM1H S124A/S211A
Plasmid#208370PurposeMammalian expression of HA-tagged human PPM1H S124A/S211ADepositorAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Δ37 PPM1H
Plasmid#207334PurposeBacterial expression of His SUMO Δ37 PPM1HDepositorAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-L2D/V9D/I19D PPM1H
Plasmid#207341PurposeMammalian expression of HA-L2D/V9D/I19D PPM1HDepositorAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only
