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pDONR223_MAP2K1_p.E203K

Plasmid

#81729

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Mutation

L197P; E203K; I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7(res) (closed)

Plasmid

#160105

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.D44N

Plasmid

#81456

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NM_005163.2 (AKT1 Human)

Use

Gateway entry vector

Mutation

D44N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R465H

Plasmid

#81571

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Mutation

R465H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Tomato MEF2C S222A

Plasmid

#89716

Purpose

Retroviral gene expression vector for MEF2C-S222A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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BAP1-C91A

Plasmid

#108440

Purpose

Stable expression of BAP1-C91A in mammalian cells.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Expression

Mammalian

Mutation

C91A: c.271T>G, c.272G>C

Promoter

CMV

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.R173H

Plasmid

#81682

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

R173H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.T182I

Plasmid

#81705

Purpose

Gateway Donor vector containing ERBB2,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

T182I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES_Tomato MEF2C S222D

Plasmid

#107231

Purpose

hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Mutation

S222D

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K4_p.S184L

Plasmid

#81681

Purpose

Gateway Donor vector containing MAP2K4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K4 (MAP2K4 Human)

Use

Gateway entry vector

Mutation

S184L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.E62K

Plasmid

#81656

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

E62K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.G12A

Plasmid

#81277

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

G12A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.I139F

Plasmid

#81627

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

I139F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.T331M

Plasmid

#81450

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

T331M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.A47V

Plasmid

#81455

Purpose

Gateway Donor vector containing IDH2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

A47V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.A416V

Plasmid

#81443

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

A416V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.E268D

Plasmid

#81403

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

E268D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.G137E

Plasmid

#81407

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

G137E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJC2527 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-StrepII-FBXO31(∆1-65)

Plasmid

#250252

Purpose

Recombinant production of SKP1(A2P, ∆38-43, ∆71-82) and 6His-Smt3-StrepII-FBXO31(∆1-65).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Inserts

SKP1 (SKP1 Human)
FBXO31 (FBXO31 Human)

Tags

6xHis-Smt3(SUMO)-StrepII

Expression

Bacterial

Mutation

A2P, ∆38-43, ∆71-82 and ∆1-65

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pHoY108_pAC8_hsDCAF5_1-477

Plasmid

#249181

Purpose

Express DCAF5(1-477) in Insect cells

Depositor

Eric Fischer

Article

Radko-Juettner et al Nature. 2024 Apr;628(8007):442-449. doi:

Insert

DCAF5(1-477) (DCAF5 Human)

Tags

Strep_BirA_short-linker-TEV

Expression

Insect

Mutation

Truncation 1-477

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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