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pCS2+8-asp-2-flag-tev-halo-his

Plasmid

#227153

Purpose

For T-REX experiments of asp-2.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

asp-2 (asp-2 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSE89: pFuGW-G5p-EYFP

Plasmid

#240461

Purpose

Synthetic GAL4p comprising 5 tandem GAL4 binding sites (G5p) regulating EYFP

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

G5p
EYFP

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE92: pFuGW-G14p-EYFP

Plasmid

#240462

Purpose

A synthetic GAL4 promoter comprising 14 tandem GAL4 binding sites (G14p) regulating EYFP

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

EYFP
G14p

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE152: pFuGW-hUbCp-3CLpro-SV40NLS

Plasmid

#240455

Purpose

SARS-CoV-2 Protease 3CLpro expression plasmid

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Insert

3CLpro

Use

Lentiviral

Promoter

hUbCp

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Merlin(dog)gRNA1

Plasmid

#214823

Purpose

A knockout vector for dog Merlin.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A gRNA targeting the dog NF2(Merlin) gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-Merlin(dog)gRNA2

Plasmid

#214824

Purpose

A knockout vector for dog Merlin.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A gRNA targeting the dog NF2(Merlin) gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-chGrb2/eDHFR(69K6)-iRFP713

Plasmid

#214828

Purpose

Encoding Grb2-eDHFR(69K6) chimera fused to iRFP713

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713

Tags

iRFP713

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-iS-methadoneSnFR-Golgi

Plasmid

#220865

Purpose

Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR

Depositor

Henry Lester

Article

Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

Insert

iS-methadoneSnFR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12-Clover

Plasmid

#215527

Purpose

Expression vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12

Plasmid

#215517

Purpose

Dox-inducible lentiviral transfer vector with PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12-Clover

Plasmid

#215521

Purpose

Gateway entry vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(E378G)

Plasmid

#215462

Purpose

Gateway entry vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

E378G (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-VPS35-mScarlet

Plasmid

#215472

Purpose

Gateway entry vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Use

Gateway entry vector

Tags

mScarlet

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(Y527F)

Plasmid

#215460

Purpose

Gateway entry vector with Src constitutively-active mutant (Y527F)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

Y527F (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(K295R)

Plasmid

#215461

Purpose

Gateway entry vector with Src kinase-dead mutant (K295R)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

K295R (Kinase dead)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE2-EF1alpha TDH

Plasmid

#236457

Purpose

stable expression of TDH using viral infection

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

TDH (Tdh Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Strep(II)(N)-PIN1

Plasmid

#231724

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

PIN1 (PIN1 Human)

Tags

Strep(II)-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RFP-SIKE

Plasmid

#235691

Purpose

Express RFP tagged SIKE gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SIKE (SIKE1 Human)

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ HA-BEX1

Plasmid

#231002

Purpose

Transient overexpression of human BEX1 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX1 (BEX1 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-N1(1-445)

Plasmid

#225464

Purpose

Express mEGFP-fusion protein of FMR1 fragment (1-445)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N1 fragment (1-445)

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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