We narrowed to 16,535 results for: grna

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MDH1-pre-let-7_PShairpin

Plasmid

#26537

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

pre-let-7_PS hairpin

Use

Retroviral

Expression

Mammalian

Mutation

5 nt mutated to make pre-let-7 stem pairing perfe…

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfRab8b_1

Plasmid

#26708

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8b RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
lentiCRISPR v2

Plasmid

#52961

Purpose

Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-yA

Plasmid

#191015

Purpose

CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

Depositor

Perng-Kuang Chang

Article

Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

Insert

yA

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_011

Plasmid

#59702

Purpose

This lentiviral vector can be used to assay Cas9 activity.

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

EGFP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Has Service

Cloning Grade DNA

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRCA594 - pBA904 Puro-T2A-BFP

Plasmid

#238170

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEcCas

Plasmid

#73227

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

Depositor

Sheng Yang

Article

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Inserts

Cas9
sgRNA

Use

CRISPR; Red recombinase

Expression

Bacterial

Promoter

native promoter

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLP16_Lenti Scramble Control

Plasmid

#239417

Purpose

Negative control Lentiviral plasmid for SpCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble sequence

Use

Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CROPseq-Puro-4xMS2

Plasmid

#212626

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-shBRCA1 #2

Plasmid

#44595

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

BRCA1 (Brca1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pCAS9c

Plasmid

#141250

Purpose

Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Article

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1 (Cas9), pSNR52 (gRNA)

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-2

Plasmid

#129727

Purpose

Expressing SpCas9 and sgAAVS1-2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-2

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pACRISPR

Plasmid

#113348

Purpose

A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Aurora A

Plasmid

#198402

Purpose

Expression of EGFP-tagged Aurora A in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Aurora A

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_213

Plasmid

#133456

Purpose

H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

Control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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