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LLPS-pCDH-DDX6-NT

Plasmid

#240015

Purpose

Expresses human DDX6-NT fused to EGFP in mammalian cells

Depositor

Rui Su

Publication

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-NT (1-302)

Use

Lentiviral

Tags

6xHis and EGFP

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pRO275

Plasmid

#233825

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-1XOaf1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-1XOaf1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO276

Plasmid

#233826

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN102

Plasmid

#233828

Purpose

MoClo-YTK; type4A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO211

Plasmid

#233830

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pTDH3-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO261

Plasmid

#233831

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pRPL18B-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO262

Plasmid

#233832

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pRNR2-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO263

Plasmid

#233833

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pRAD27-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRAD27-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO264

Plasmid

#233834

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pREV1-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pREV1-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO230

Plasmid

#233837

Purpose

MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA3-tADH1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-rtTA3-tADH1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO254

Plasmid

#233842

Purpose

MoClo-YTK; ConLS/R1 cassette; rtTA systems; pTDH3-rtTA3-tADH1

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-rtTA3-tADH1

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS

Plasmid

#233920

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS

Depositor

Marcus Taylor

Publication

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-1x

Plasmid

#233923

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif

Depositor

Marcus Taylor

Publication

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-1x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3x

Plasmid

#233925

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs

Depositor

Marcus Taylor

Publication

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-3x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-5x

Plasmid

#233927

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs

Depositor

Marcus Taylor

Publication

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-5x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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hPDZD11-HA

Plasmid

#236537

Purpose

For expression of PDZD11 (human) in mammalian cells

Depositor

Sandra Citi

Publication

Guerrera et al J Biol Chem. 2016 May 20;291(21):11016-2

Insert

PDZD11 (PDZD11 Human)

Tags

HA

Expression

Mammalian

Mutation

WT

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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p185-RV-CAG-dio-mScarlet-T2A

Plasmid

#204739

Purpose

Conditional expression of target gene with fluorescent reporter mScarlet

Depositor

Lynette Lim

Publication

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Type

Empty backbone

Use

Retroviral

Promoter

CAG

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Publication

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFNC-8

Plasmid

#227670

Purpose

Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.

Depositor

Pablo Ivan Nikel

Publication

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Insert

AmR

Expression

Bacterial

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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