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pGEX2T-TEV CHMP1B 4-178

Plasmid

#108284

Purpose

Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

Depositor

Wesley Sundquist

Publication

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 179-199

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAG660 lyn11-pFAST

Plasmid

#172863

Purpose

expresses lyn11-pFAST in mammalian cells (inner membrane labeling)

Depositor

Arnaud Gautier

Publication

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

Lyn11-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-D-kcnj1a.1

Plasmid

#173125

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Publication

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj1a (kcnj1a.1 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

Plasmid

#162964

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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VP2C-GFP-MIOX only

Plasmid

#166684

Purpose

Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Publication

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

VP2C-GFP-MIOX

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL10

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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MIOX only

Plasmid

#166681

Purpose

Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Publication

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

MIOX (Miox Mouse, Synthetic, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL10

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGB_ctail_pET28

Plasmid

#167330

Purpose

Expresses TFAM HMGB+C-tatil mutant in bacterial cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGB and C-tail doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG(C104A/M247C)

Plasmid

#155133

Purpose

Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Publication

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

C104A/M247C, insert codes for GlpG residues 87-276

Promoter

T7

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-K283)-FRB

Plasmid

#154451

Purpose

expresses flag-pBirA(1-K283)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-K283)-FRB

Expression

Mammalian

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1_cryst

Plasmid

#133271

Purpose

X. laevis expression vector. It will generate the TREK-1 channel

Depositor

Dan Minor

Publication

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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prNFL-P22S-EGFP

Plasmid

#132594

Purpose

Expresses hEGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutation

Depositor

Anthony Brown

Publication

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

64C>A, g5C>G, Pro22Ser

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127399

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Publication

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k

Plasmid

#127408

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Publication

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127405

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Publication

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCuHA-ATG13[3SD](426)

Plasmid

#122077

Purpose

Expressing HA-ATG13[3SD]

Depositor

Daniel Klionsky

Publication

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to aspartic acid; Serine 649 to aspart…

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-ATG13[3SD](315)

Plasmid

#122074

Purpose

Expressing GFP-Atg13[3SD]

Depositor

Daniel Klionsky

Publication

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Mutation

Serine 646 to aspartic acid; Serine 649 to aspart…

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoA

Plasmid

#117990

Purpose

Chloroplast-targeted expression of CURT_fluoA controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Publication

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoA

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoB

Plasmid

#117991

Purpose

Chloroplast-targeted expression of CURT_fluoB controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Publication

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoB

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoE

Plasmid

#117992

Purpose

Chloroplast-targeted expression of CURT_fluoE controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Publication

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoE

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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