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C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

Plasmid

#162933

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-maltose binding protein fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

Plasmid

#162932

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion with AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E21: attB4-PGKp>-attB1r

Plasmid

#162921

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E07: attB2r-HA-attB3

Plasmid

#162911

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

HA epitope tag

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSC8

Plasmid

#50428

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC7

Plasmid

#50427

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC6

Plasmid

#50426

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC5

Plasmid

#50425

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC4

Plasmid

#50424

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC3

Plasmid

#50423

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC2

Plasmid

#50422

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC10

Plasmid

#50430

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.

Depositor

Andrew Scharenberg

Publication

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pYJA5

Plasmid

#217778

Purpose

The empty vector for quadruple sgRNA cloning

Depositor

Adriano Aguzzi

Publication

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Puromycin resistance gene, T2A, and TagBFP

Expression

Mammalian

Promoter

PGK, CMV, U6

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

Plasmid

#210523

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Publication

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA-directcapture

Use

AAV

Expression

Mammalian

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUW-hTie1-FLAG

Plasmid

#119233

Purpose

Plasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.

Depositor

Kari Alitalo

Insert

TIE1 (TIE1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

P hUbC

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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cwby0005

Plasmid

#232214

Purpose

GGA cloning MS-his-AGGA-promoter-ccdb-TTCC

Depositor

David Baker

Publication

Qian et al Nat Commun. 2026 Aug 20;17(1):9945. doi:

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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cwby0002

Plasmid

#232211

Purpose

GGA cloning MSG-AGGA-promoter-ccdb-TTCC-HIS

Depositor

David Baker

Publication

Qian et al Nat Commun. 2026 Aug 20;17(1):9945. doi:

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pfa6a PhoCl-TsCC(B)::KANMX

Plasmid

#185906

Purpose

Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

Depositor

Matthew Good

Publication

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

PhoCl-TsCC(B)

Tags

PhoCl fluorophore with C-terminal TsCC(B)

Expression

Yeast

Promoter

SP6

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173864

Purpose

Mammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Publication

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mVenus-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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