We narrowed to 17,223 results for: EMB
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Plasmid#169022PurposeExpresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteriaDepositorInsertp97
TagsHisExpressionBacterialMutationD592TAGPromoterT7Available SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r
Plasmid#162950PurposeGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed proteinDepositorInsertdual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
UseSynthetic BiologyAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyExpressionYeastPromoterGAL10Available SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 only
Plasmid#166675PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertMurine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyExpressionYeastPromoterGAL1Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700]-Ss(424)
Plasmid#166852PurposeExpresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.DepositorAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
TagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)
Plasmid#155136PurposeExpresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1DepositorInsertGlpG (glpG Escherichia coli)
TagsGST and HAExpressionBacterialMutationD243G/L244G/F245G/M247G/S248G/M249G/A250GPromotertacAvailable SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pBirA(1-K283)-FRB
Plasmid#154451Purposeexpresses flag-pBirA(1-K283)-FRB/biochemical assayDepositorInsertflag-pBirA(1-K283)-FRB
ExpressionMammalianPromoterT7 promoterAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) C117S
Plasmid#108915PurposeExpress C117S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
TagsHalo-TEVExpressionMammalianMutationCysteine 117 to SerinePromoterSP6Available SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9n-Nanog-R
Plasmid#122303PurposeExpresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Nanog (Nanog Mouse, Synthetic)
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB0238-TEV-His12
Plasmid#137038Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66635.2
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmApple-EGFP-HRas
Plasmid#133854PurposeMammalian expression of HRas N-terminally tagged with mApple and EGFPDepositorAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TIRAP-cGASΔN-HA
Plasmid#130921PurposeExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection markerDepositorInsertTIRAP-ΔNcGAS
TagsHAExpressionMammalianMutationcGAS N terminus (aa1-159) replaced with the N ter…PromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37delta2-81
Plasmid#113502PurposeBacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81DepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 2-81Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL His-I3
Plasmid#113513PurposeExpression of His-I3 in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
NES-PAmCherry-cPHx1
Plasmid#116859PurposePI(3,4)P2 biosensorDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#84
Plasmid#110878PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neuronsDepositorInsertsunc-17beta promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialMutationChanged Proline to Serine at amino acid 260Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
ExpressionBacterialAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#81
Plasmid#110876PurposeExpresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialMutationChanged Proline to Serine at amino acid 260Available SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#83
Plasmid#110877PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialMutationChanged Proline to Serine at amino acid 260Available SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only