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pPA-RL-FL

Plasmid

#78863

Purpose

DULIP Tandem Construct used for data normalization

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

Firefly

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCheck2 Dest (R1-R2) #BGV129

Plasmid

#48955

Purpose

Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Luciferase; Gateway cloning vector

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmNot10

Plasmid

#79254

Purpose

Expresses EGFP-tagged DmNot10 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot10 (Not10 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot11

Plasmid

#79253

Purpose

Expresses EGFP-tagged DmNot11 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot11 (Not11 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_K5S

Plasmid

#98679

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-rat Fcepsilon RI gamma

Plasmid

#8375

Depositor

Jean Kinet

Article

Blank et al Nature 1989 Jan 12;337(6203):187-9.

Insert

rat Fc epsilon RI gamma cDNA (Fcer1g Rat)

Use

Beta-gal

Expression

Bacterial

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
myc-SpELL pBacPAK8

Plasmid

#15318

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-SpEAF 60-251 pBacPAK8

Plasmid

#15321

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

EAF1 (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Mutation

N terminal deletion lacking first 59 amino acids …

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEF1-Pls2-sfGFP

Plasmid

#205749

Purpose

Expresses sfGFP under IPTG inducible promoter Pls2 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/low strength.

Depositor

Jerome Bonnet

Article

Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

Type

Empty backbone

Use

Shuttle vector gram+ gram-

Expression

Bacterial

Promoter

Pls2 (Phyperspank mutant, IPTG inducible)

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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