Skip to main content
Addgene
Showing: 1101 - 1120 of 1250 results

  1. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

    Enlarge

  9. NODALvar-CAG-AAVS1

    Plasmid
    #115262
    Purpose
    For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115262-plasmid-map-sequence-id-222505

    Enlarge

  19. AAVS1-TRE3G-NODALvar-matMYC

    Plasmid
    #115641
    Purpose
    For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115641-plasmid-map-sequence-id-232862

    Enlarge

  20. NODAL-MYC-DYK-N72A-N199A

    Plasmid
    #115252
    Purpose
    For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N72A, N199A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115252-plasmid-map-sequence-id-222234

    Enlarge

Showing: 1101 - 1120 of 1250 results