We narrowed to 1,138 results for: fret

1. pcDNA3-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123329

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

   Plasmid

   #123339

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Venus-cpVenus-FLARE-CKAR

   Plasmid

   #123343

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

   Plasmid

   #123336

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-cpCerulean3-FLARE-AKAR
   

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-ICUE4

   Plasmid

   #181846

   Purpose

   Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   ICUE4 (RAPGEF3 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV x EF1a_EKAREN4-ires-puro

   Plasmid

   #167828

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-NES-ZapCV2

   Plasmid

   #222950

   Purpose

   Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2 (NES Synthetic)
   

   Tags

   ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV x EF1a_EKAREN5-ires-puro

   Plasmid

   #167821

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV x EF1a_EKAREN5-ires-blast

   Plasmid

   #167822

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV x EF1a_EKAREN4-ires-blast

    Plasmid

    #167829

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TdLanYFP-AURKA-Aquamarine

    Plasmid

    #228562

    Purpose

    Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

    Depositor

    Giulia Bertolin

    Article

    Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

    Insert

    AURKA (AURKA, AURKA Human)
    

    Tags

    Aquamarine and TdLanYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-Pur-Lyn-AktAR2-D

    Plasmid

    #125198

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    Lyn-AktAR2-D
    

    Use

    Transposon

    Tags

    Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-YFP-KRAS4B

    Plasmid

    #112718

    Purpose

    Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    YFP-KRAS4B (KRAS Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CFP-KRAS4B

    Plasmid

    #112717

    Purpose

    Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    CFP-KRAS4B (KRAS Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5/FRT/TO_COX8-Halo-PanK

    Plasmid

    #197949

    Purpose

    Expression of FRET_control in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-ecPanK
    

    Expression

    Mammalian

    Mutation

    PanK=PanK-D127A,L277W,F252Y

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT/TO_Halo-PanK

    Plasmid

    #197948

    Purpose

    Expression of FRET_control in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-ecPanK
    

    Expression

    Mammalian

    Mutation

    PanK=PanK-D127A,L277W,F252Y

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(−) mCER-Prx2

    Plasmid

    #158224

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Expression

    Mammalian

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-SUMO mCER-Prx2

    Plasmid

    #158226

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Expression

    Bacterial

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPCX mCER-Prx1

    Plasmid

    #158227

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx1
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLPCX mCER-Prx2

    Plasmid

    #158225

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge