We narrowed to 5,868 results for: nls

1. DsRed-CPI-17-T38E-NLS

   Plasmid

   #100790

   Purpose

   Mammalian expression of Fluorescent CPI-17 phospho mimetic nuclear

   Depositor

   Joseph Gordon

   Insert

   CPI-17-T38E-NLS (PPP1R14A Human)
   

   Use

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   T38E

   Promoter

   CMV

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TTmiRc2 3XFLAG-EGFP-NLS

   Plasmid

   #87778

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

   Plasmid

   #87777

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK7-EE
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

   Plasmid

   #87776

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4 (MAP2K4 Human)
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-myc-mCE NLS

   Plasmid

   #82465

   Purpose

   Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   Capping Enzyme (Rngtt Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   deleted amino acids Nuclear Localization Signal

   Promoter

   CMV

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-3xFLAG-NLS-TPP1-OB

   Plasmid

   #79060

   Purpose

   Retroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domain

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TPP1 (TPP1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Wild-type TPP-OB folding domain

   Promoter

   CMV

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pLPC-Flag-CHES1L-dC-NLS

   Plasmid

   #51840

   Purpose

   Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

   Depositor

   Gerardo Ferbeyre

   Article

   Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

   Insert

   CHES1 (FOXN3 Human)
   

   Use

   Retroviral

   Tags

   Flag and NLS

   Expression

   Mutation

   contains CHES1 amino acids 1-205

   Promoter

   Available since

   July 17, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLPC-Flag-CHES1LdFC-NLS

   Plasmid

   #51838

   Purpose

   Flag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS

   Depositor

   Gerardo Ferbeyre

   Article

   Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

   Insert

   CHES1 (FOXN3 Human)
   

   Use

   Retroviral

   Tags

   Flag and NLS

   Expression

   Mutation

   contains CHES1 amino acids 1-109

   Promoter

   Available since

   July 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. NLS-PyronicSF/pCMV-myc-nuc

   Plasmid

   #124814

   Purpose

   Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

   Depositor

   Luis Felipe Barros

   Article

   Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

   Insert

   PyronicSF
   

   Use

   Tags

   Nuclear Localization Signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRM055 His-TEV-Cas9 6xNLS 0C

    Plasmid

    #196246

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha and IRES

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. phage UbiC NLS HA stdMCP Halo

    Plasmid

    #104098

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    stdMCP-Halo
    

    Use

    Lentiviral

    Tags

    C-terminal Halo-tag, N-terminal NLS, and Two syno…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

    Plasmid

    #218793

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    NLSf-mScarletP2A-hLuc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

    Plasmid

    #31484

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    EF1s HA NLS Sce(opt) T2A IFP1.4
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    "Opt" indicates the I-SceI coding seque…

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. phage UbiC NLS HA stdPCP stdGFP

    Plasmid

    #104099

    Purpose

    Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    stdPCP-stdGFP
    

    Use

    Lentiviral

    Tags

    N-terminal NLS, Two synonymous copies of GFP (C-t…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid

    #212196

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

    Depositor

    Stacey Edwards

    Article

    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

    Insert

    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    

    Use

    CRISPR and Lentiviral

    Tags

    HA, NLS, and blasticidin S deaminase

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. phage UbiC NLS HA stdMCP stdHalo

    Plasmid

    #104999

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    stdMCP-stdHalo
    

    Use

    Lentiviral

    Tags

    HA tag, NLS, Two synonymous copies of the Halo-ta…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. phage-ubc-nls-ha-tdPCP-gfp

    Plasmid

    #40650

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    tdPCP-gfp
    

    Use

    Lentiviral

    Tags

    EGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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