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Plasmid#148673PurposeBacterial Expression of HsNot1_1093-1588DepositorInsertHsNot1_1093-1588 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot1iso1_1351-1588_AE
Plasmid#148630PurposeBacterial Expression of HsNot1iso1_1351-1588DepositorInsertHsNot1iso1_1351-1588 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot1iso1_1356-1581_V
Plasmid#147779PurposeBacterial Expression of HsNot1iso1_1356-1581DepositorInsertHsNot1iso1_1356-1581 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
ORF18(R158A_L159A)-3xFLAG pCDNA4
Plasmid#129814PurposeExpresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Arginine 158 to Alanine and Leucine 159 t…PromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(R158A)-3xFLAG pCDNA4
Plasmid#129801PurposeExpresses KSHV ORF18 (R158A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Arginine 158 to AlaninePromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 Y158F
Plasmid#123624PurposeExpresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationTyr158PhePromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-3158-3p
Plasmid#103428PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-3158-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-3158-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-3158-5p
Plasmid#103429PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-3158-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-3158-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_W158A/pET28b(+) N-tag
Plasmid#71358Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:W158ADepositorInsertHP0518 (HP0518 changed Tryptophan 158 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158) in pcDNAI/Amp
Plasmid#60895PurposeThis vector attaches CFP(1-158) to the N-terminus of a protein.DepositorInsertCFP(1-158)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme W126Y W138Y W158Y
Plasmid#18559DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationW126Y W138Y W158YPromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
Anti-Histone H3.3 [N158/28R]
Plasmid#182106PurposeMammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.DepositorInsertanti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-1 in pcDNAI/Amp
Plasmid#54468PurposeAn amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP (1-158)/beta-1 (GNB1 Aequorea victoria, Human)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-AsLOV2-NES27 (pDN158)
Plasmid#72659PurposeConstitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear exportDepositorInsertH2B-GFP-AsLOV2-NES27
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA Oct4A -158
Plasmid#50922PurposeU6 driven sgRNA targeting OCT4 isoform A -158 bp from TSSDepositorInsertOct4A -158 sgRNA (POU5F1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-beta-5 in pcDNAI/Amp
Plasmid#55708PurposeAn amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertmCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-5 was amplified via PCR, which added an N-t…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-2 in pcDNAI/Amp
Plasmid#54469PurposeAn amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP(1-158)/beta-2 (GNB2 Aequorea victoria, Human)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationYFP (1-158) includes a substitution of Met for Gl…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-5 in pcDNAI/Amp
Plasmid#55625PurposeAn amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-5 (GNG5 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-5 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-5 in pcDNAI/Amp
Plasmid#54470PurposeAn amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP(1-158)/beta-5 (GNB5 Aequorea victoria, Human)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationYFP (1-158) includes a substitution of Met for Gl…PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF138-delta 158-180
Plasmid#78923PurposeMammalian expression of RNF138 with delta 158-180 and an EGFP fusionDepositorInsertRNF138 (RNF138 Human)
UseTagsEGFPExpressionMammalianMutationdelta 159-180PromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only