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Showing: 1101 - 1120 of 9744 results
  1. pnYC-NpM-HsNot1_1093-1588_AF

    Plasmid
    #148673
    Purpose
    Bacterial Expression of HsNot1_1093-1588
    Depositor
    Insert
    HsNot1_1093-1588 (CNOT1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. ORF18(R158A_L159A)-3xFLAG pCDNA4

    Plasmid
    #129814
    Purpose
    Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells
    Depositor
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Arginine 158 to Alanine and Leucine 159 t…
    Promoter
    CMV
    Available since
    Oct. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. LSB-hsa-miR-3158-3p

    Plasmid
    #103428
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-3158-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Insert
    hsa-miR-3158-3p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. LSB-hsa-miR-3158-5p

    Plasmid
    #103429
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-3158-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Insert
    hsa-miR-3158-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. T4 Lysozyme W126Y W138Y W158Y

    Plasmid
    #18559
    Depositor
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    W126Y W138Y W158Y
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only
  6. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    YFP (1-158)/beta-1 (GNB1 Aequorea victoria, Human)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. Cer(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #55708
    Purpose
    An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    mCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-5 was amplified via PCR, which added an N-t…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid
    #54469
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    YFP(1-158)/beta-2 (GNB2 Aequorea victoria, Human)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. Cer(1-158)-gamma-5 in pcDNAI/Amp

    Plasmid
    #55625
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Insert
    Cer(1-158)-gamma-5 (GNG5 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-5 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #54470
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Insert
    YFP(1-158)/beta-5 (GNB5 Aequorea victoria, Human)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1101 - 1120 of 9744 results