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pEPYC1CB0305

Plasmid

#154625

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_285 reporters [MinSyn_285::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0503

Plasmid

#154628

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_000 reporters [MinSyn_000::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0477

Plasmid

#154626

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_104 reporters [MinSyn_104::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYC1CB0478

Plasmid

#154627

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_108 reporters [MinSyn_108::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-AlkB

Plasmid

#228218

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Junchao Shi

Article

Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

Insert

Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)

Use

Tags

His tag

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE

Plasmid

#188763

Purpose

Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

NCOA3 (NCOA3 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

Plasmid

#177868

Purpose

Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

mGreenLantern-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

OLLAS

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRFP

Plasmid

#125778

Purpose

constitutive expression of a short-hairpin RNA targeting RFP

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_FLuc_loxP-PuroR

Plasmid

#177866

Purpose

Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

FLuc-based EXSISERS

Use

CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJT222 lenti pEF-rTetR(SE-G72P)-3XFLAG-FOXO3 FOXO-TAD-T2A-mCherry-BSD-WPRE

Plasmid

#188760

Purpose

Lentiviral backbone containing FOXO3 FOXO-TAD as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

FOXO3 FOXO-TAD (FOXO3 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJT226 lenti pEF-rTetR(SE-G72P)-3XFLAG-SMARAC2 QLQ-T2A-mCherry-BSD-WPRE

Plasmid

#188761

Purpose

Lentiviral backbone containing SMARCA2 QLQ as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

SMARCA2 QLQ (SMARCA2 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJT228 lenti pEF-rTetR(SE-G72P)-3XFLAG-KIBRA WW-T2A-mCherry-BSD-WPRE

Plasmid

#188762

Purpose

Lentiviral backbone containing KIBRA WW as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

KIBRA WW (WWC1 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJT236 lenti pEF-rTetR(SE-G72P)-3XFLAG-CXXC1 PHD-T2A-mCherry-BSD-WPRE

Plasmid

#188764

Purpose

Lentiviral backbone containing CXXC1 PHD as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

CXXC1 PHD (CXXC1 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMV5 lenti pEF-rTetR(SE-G72P)-3XFLAG-APBB1(WW)-T2A-mCherry-BSD-WPRE

Plasmid

#188751

Purpose

Lentiviral backbone containing APBB1 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

APBB1 WW (APBB1 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV6 lenti pEF-rTetR(SE-G72P)-3XFLAG-CRTC2(TORC-C)-T2A-mCherry-BSD-WPRE

Plasmid

#188752

Purpose

Lentiviral backbone containing CRTC2 TORC_C domain as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

CRTC2 TORC_C (CRTC2 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMV7 lenti pEF-rTetR(SE-G72P)-3XFLAG-CACO1(Zn-C2H2_12)-T2A-mCherry-BSD-WPRE

Plasmid

#188753

Purpose

Lentiviral backbone containing CACO1 Zn-C2H2_12 domain as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

CACO1 Zn-C2H2_12 (CALCOCO1 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEXSISERS_NLuc_FRT-PuroR

Plasmid

#177865

Purpose

Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

NLuc-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

OLLAS

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEXSISERS_BSD_FRT-PuroR

Plasmid

#177867

Purpose

Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

BSD-based EXSISERS

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

OLLAS

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEXSISERS_SurfaceHaloTag_FRT-PuroR

Plasmid

#177869

Purpose

Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

Surface-HaloTag-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Use

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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