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1029-AIDx-CP

Plasmid

#135341

Purpose

Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

CP 1029 nSpyCas9-hAIDx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pECE-HA-FOXO3 S574A

Plasmid

#52426

Purpose

mammalian expression of FOX03 with mutant JNK phosphorylation site

Depositor

Steven Weinman

Article

Tikhanovich et al Hepatology. 2014 Jan;59(1):58-70. doi: 1

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S574A, mutant JNK phosphorylation site

Promoter

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

Plasmid

#61792

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-3 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pECE-HA-FOXO3 R248K R250K

Plasmid

#52427

Purpose

mammalian expression of FOXO3 methylation resistant mutant

Depositor

Steven Weinman

Article

Tikhanovich et al Hepatology. 2014 Jan;59(1):58-70. doi: 1

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

R248K R250K, mutant methylation sites

Promoter

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pPKm-235

Plasmid

#90509

Purpose

pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-sHO1, mito-Hy2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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[#GS16-LV] Syn1-MitoTRACER - Donor

Plasmid

#233565

Purpose

Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025; In Press.

Insert

MitoTRACER-Donor

Use

Lentiviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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MT2A_beta-Villin

Plasmid

#200301

Purpose

Bacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

beta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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GLRX-roGFP2 in pT3TS-Dest

Plasmid

#194297

Purpose

In vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRK

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pACE-K2P4.1-StrepTagII

Plasmid

#191474

Purpose

Expresses K2P4.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P4.1 (KCNK4 Human)

Use

Tags

Expression

Insect

Mutation

N104Q N108Q

Promoter

polH

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AfLEA1.1 (pBS0741)

Plasmid

#185233

Purpose

For the mammalian expression of the brine shrimp protein AfLEA1.1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfLEA1.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_HYPDU (pBS0650)

Plasmid

#185194

Purpose

For the mammalian expression of the tardigrade protein CAHS1_HYPDU. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_HYPDU

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_HUMAN (pBS0645)

Plasmid

#185190

Purpose

For the mammalian expression of the human protein APOA4_HUMAN. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_HUMAN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_A0A1D1UKG0_RAMVA (pBS0352)

Plasmid

#185188

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UKG0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UKG0_RAMVA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_B1PM76_ARTSF (pBS0329)

Plasmid

#185176

Purpose

For the mammalian expression of the brine shrimp protein B1PM76_ARTSF. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

B1PM76_ARTSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_B9VQB0_ARTSF (pBS0327)

Plasmid

#185175

Purpose

For the mammalian expression of the brine shrimp protein B9VQB0_ARTSF. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

B9VQB0_ARTSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_A6YT97_ARTSF (pBS0322)

Plasmid

#185172

Purpose

For the mammalian expression of the brine shrimp protein A6YT97_ARTSF. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A6YT97_ARTSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_A0A1D1UJP0_RAMVA (pBS0306)

Plasmid

#185166

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UJP0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UJP0_RAMVA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_BnHD (pBS0280)

Plasmid

#185155

Purpose

For the mammalian expression of the rapeseed protein BnHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

BnHD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_CAHS1_RAMVA (pBS0267)

Plasmid

#185150

Purpose

For the mammalian expression of the tardigrade protein CAHS1_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_RAMVA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge