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Plasmid#109793PurposeProtein expression and purification of ARPC3_P21-ArcDepositorInsertARPC3_P21-Arc (ARPC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-Fc-His
Plasmid#72189PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.26
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.23 EC10-Fc-His
Plasmid#72187PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.23
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-AP-His
Plasmid#72063PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.26
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ATP5
Plasmid#109857PurposeProtein expression and purification of ATP5DepositorInsertATP5 (ATP5J Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
UseTags6His-TEVExpressionBacterialMutationR47APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
UseTags6His-TEVExpressionBacterialMutationR47KPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
UseTags6His-TEVExpressionBacterialMutationQ48APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
UseTags6His-TEVExpressionBacterialMutationT50APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Thy-C73A-HA
Plasmid#111542PurposeFor expression of human KCNB1 in mouse brainDepositorInsertthy1.2-C73A-HA (KCNB1 Human)
UseMouse TargetingTagsHAExpressionMutationC73APromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plxnb1(S)-Fc-His
Plasmid#72127PurposeExpresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnb1 (Plxnb1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.6-Fc-His
Plasmid#72103PurposeExpresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNrp2.6 (Nrp2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.2-Fc-His
Plasmid#72099PurposeExpresses the extracellular region of the Neuropilin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNrp2.2 (Nrp2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.e-Fc-His
Plasmid#72114PurposeExpresses the extracellular region of the Netrin G1, isoform e protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtng1.e (Ntng1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.g-Fc-His
Plasmid#72116PurposeExpresses the extracellular region of the Netrin G1, isoform g protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtng1.g (Ntng1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng1.d-Fc-His
Plasmid#72113PurposeExpresses the extracellular region of the Netrin G1, isoform d protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtng1.d (Ntng1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntng2.b-Fc-His
Plasmid#72121PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtng2.b (Ntng2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(L,-)-Fc-His
Plasmid#72148PurposeExpresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3f (Sema3f Mouse)
UseTagsFc-HisExpressionMammalianMutationV319IPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3b(L)-Fc-His
Plasmid#72139PurposeExpresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3b (Sema3b Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(S,-)-Fc-His
Plasmid#72150PurposeExpresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3f (Sema3f Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only