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pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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DT043A

Plasmid

#159404

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T7-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

T7 bacteriophage promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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776 pME-miR-126

Plasmid

#26033

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

miR-126 (mir126a Zebrafish)

Use

Entry vector

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only
pMEclecl14aP1gfp

Plasmid

#90123

Purpose

Gateway Mid-entry clone containing promoter sequence specific for clec14a driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aP1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5Eclec14aE1

Plasmid

#90117

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of clec14a

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-CFP-HA no-pA

Plasmid

#82591

Purpose

3' entry vector containing HA epitope fused to c-terminus of CFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

ECFP-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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C512-E04: attB5r-mCherry-nostop-attB1r

Plasmid

#162941

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA55

Plasmid

#174768

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E without STOP codon

Use

Unspecified

Mutation

L17E

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA56

Plasmid

#174769

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V without STOP codon

Use

Unspecified

Mutation

L17E/D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA54

Plasmid

#174767

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V without STOP codon

Use

Unspecified

Mutation

D481V

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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