We narrowed to 18,078 results for: comp

1. pTYB11-PTBP1-RRM2CCtoSS

   Plasmid

   #89154

   Purpose

   recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
   

   Use

   Tags

   Intein and chitin binding domain

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. FH1-MGT#2-mCherry_PGK-H2B-CFP

   Plasmid

   #164102

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   MGT#2-mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBT9-Hook3

   Plasmid

   #198525

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook3 (HOOK3 Human)
   

   Use

   Tags

   GAL4-DNA binding domain (BD) fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO HSPH2

   Plasmid

   #19454

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 4 (HSPA4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations A381T, T732A, and an insertion of "…

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. shBIRC5 # 2

   Plasmid

   #42554

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shBIRC5 (BIRC5 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

6. Cer(1-158)-RGS7

   Plasmid

   #55760

   Purpose

   An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
   

   Use

   Tags

   Cer(1-158) was fused to the amino terminus of RGS…

   Expression

   Mammalian

   Mutation

   RGS7 was amplified via PCR, which added an N-term…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEBTet-SNAP-Cep97

   Plasmid

   #136800

   Purpose

   Mammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep97 (CEP97 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. APEX2-6xHp

   Plasmid

   #214411

   Purpose

   Lipid droplet localization of APEX2-6xHp for electron microscopy

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   6x Spastin aa 43-92 (APEX2 Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. shBIRC5 # 1

   Plasmid

   #42553

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shBIRC5 (BIRC5 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rab8-T22N(Flag) in pcDNA3.1neo

    Plasmid

    #46784

    Purpose

    expression of T22N Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A T22N (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T22N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-nEF-NpHR3.3-EYFP

    Plasmid

    #137151

    Purpose

    Viral expression of NpHR3.3-EYFP

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    NpHR3.3-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    W179F

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF073 MBP-Axin-His

    Plasmid

    #154011

    Purpose

    Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-ZIP(WT)

    Plasmid

    #27134

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. KAT6B-Tol2

    Plasmid

    #113384

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Expression

    Mutation

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. myc-Atg14 (396-492)

    Plasmid

    #86755

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 396-492

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. myc-Atg14 (181-413)

    Plasmid

    #86754

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 181-413

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. myc-Atg14 (201-492)

    Plasmid

    #86751

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 201-492

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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