161,547 results

1. pYPQAT07

   Plasmid

   #223379

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

   Plasmid

   #235150

   Purpose

   Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   HaloTag linked to SpyCatcher3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

   Plasmid

   #231372

   Purpose

   Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

   Depositor

   David Bartel

   Article

   Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

   Insert

   Human Argonaute2 (AGO2 Synthetic, Human)
   

   Use

   Tags

   3xFLAG-SUMO_Eu1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMC00035 (aka pMG0784)

   Plasmid

   #210768

   Purpose

   Lentiviral vector to express human ADRM1

   Depositor

   Raj Chari

   Article

   Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

   Insert

   ADRM1 (ADRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBWB514

   Plasmid

   #209326

   Purpose

   p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.

   Depositor

   Adam Arkin

   Article

   Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCambia-dLB-kanR-GFP

   Plasmid

   #223177

   Purpose

   Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

   Depositor

   Andreas Stuermer

   Type

   Empty backbone

   Use

   Ti plasmid for agrobacterium

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBES1-LacOx50-blast

   Plasmid

   #210221

   Purpose

   Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEL2319

   Plasmid

   #195697

   Purpose

   Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   Foldon-MlaC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

   Plasmid

   #178991

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEBTetD-SNAP-Cep27

    Plasmid

    #136809

    Purpose

    Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep27 (HAUS2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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