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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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hFlt1 Y1333F

Plasmid

#86037

Purpose

human VEGFR1 with Y to F mutation at position 1333

Depositor

Christie Thomas

Article

Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

Insert

human Flt1 Y1333F (FLT1 Human)

Tags

FLAG, HA, and Myc

Expression

Mammalian

Mutation

Y1333F; HA tag is inserted downstream of signal …

Promoter

CMV

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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hFLT1 delta 754-763

Plasmid

#86035

Purpose

human VEGFR1 with a 10 amino acid deletion in EC close to TM domain

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 delta754-763 aa (FLT1 Human)

Tags

FLAG, HA, and Myc

Expression

Mammalian

Mutation

delta 754-763

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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GPR148-Tango

Plasmid

#66323

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR148 (GPR148 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-Rog

Plasmid

#24429

Depositor

Bruce Conklin

Article

Scearce-Levie et al BMC Biol. 2005 Feb 11;3:3. doi: 10.1186/

Insert

RASSL (OPRK1 Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Available Since

June 16, 2010

Availability

Academic Institutions and Nonprofits only

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pACT2-DdtS-HsMCU

Plasmid

#253519

Purpose

D.discoideum targeting sequence with C-terminal tagged HsMCU mature protein in pACT2 vector

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

HsMCU (MCU Human)

Tags

FLAG

Expression

Bacterial and Yeast

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ULK1

Plasmid

#249533

Purpose

Expressing human full length ULK1

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

Flag-tag and strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pEMS2244

Plasmid

#246091

Purpose

For preclinical gene therapy: ss-AAV expression of N-term FLAG-tagged human PAX6 under control of the smCBA promoter; contains WPRE.

Depositor

Elizabeth Simpson

Article

Djaksigulova et al Gene Ther. 2026 Mar 9. doi: 10.1038/s414

Insert

ss-AAV-smCBA-3XFLAG/PAX6-WPRE (PAX6 Human)

Use

AAV

Tags

3XFLAG

Expression

Mammalian

Promoter

smCBA

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pCAD131_hIrg1_M154I_pCMV6

Plasmid

#239120

Purpose

hACOD1 with M154I mutation in mammalian expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

Myc and FLAG

Expression

Mammalian

Mutation

M154I

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5c_LxxAA (L3m)

Plasmid

#221441

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5c_LXXAA (L3m) (Fam46c Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Mutation

L332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLAS2w.Phyg-CHST8

Plasmid

#232650

Purpose

expresses CHST8 wild-type in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CHST8 (CHST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAM88

Plasmid

#226680

Purpose

Rpt1I191TAG base (3xFLAG-Rpt1I191TAG, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5, Rpt6)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

rpt1 (RPT1 Budding Yeast)
Rpt2 (RPT2 Budding Yeast)
Rpt3 (RPT3 Budding Yeast)
Rpt4 (RPT4 Budding Yeast)
Rpt5 (RPT5 Budding Yeast)
Rpt6 (RPT6 Budding Yeast)

Tags

FLAG and His

Expression

Bacterial

Mutation

I191TAG

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM89

Plasmid

#226681

Purpose

Rpt5Q49TAG base (3xFLAG-Rpt1, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5Q49TAG, Rpt6)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

rpt1 (RPT1 Budding Yeast)
Rpt2 (RPT2 Budding Yeast)
Rpt3 (RPT3 Budding Yeast)
Rpt4 (RPT4 Budding Yeast)
Rpt5 (RPT5 Budding Yeast)
Rpt6 (RPT6 Budding Yeast)

Tags

FLAG and His

Expression

Bacterial

Mutation

Q49TAG

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

Plasmid

#215910

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332D, S1346D, S1616D, S169…

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215911

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-NSD3

Plasmid

#221297

Purpose

Expression of NSD3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

NSD3 (pLxIS) (Nsd3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_V47N_I48N

Plasmid

#214319

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

V47N_I48N

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_LES33AAA

Plasmid

#214321

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

LES33AAA

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_DDEN29ENGD

Plasmid

#214320

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

DDEN29ENGD

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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