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pHH0103_TJP1-1/1-SV1/2

Plasmid

#91321

Purpose

Protein expression and purification of human SH3 domain construct TJP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP1-1/1-SV1/2 (TJP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MAP3K10-1/1

Plasmid

#91329

Purpose

Protein expression and purification of human SH3 domain construct MAP3K10-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAP3K10-1/1 (MAP3K10 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK5-1/1-IS2/2

Plasmid

#91304

Purpose

Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK5-1/1-IS2/2 (DOCK5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB2-1/2

Plasmid

#91314

Purpose

Protein expression and purification of human SH3 domain construct CACNB2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB2-1/2 (CACNB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNMBP-3/6

Plasmid

#91277

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-3/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-3/6 (DNMBP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PRAM1-1/1

Plasmid

#91279

Purpose

Protein expression and purification of human SH3 domain construct PRAM1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PRAM1-1/1 (PRAM1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK3-1/1

Plasmid

#91284

Purpose

Protein expression and purification of human SH3 domain construct DOCK3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK3-1/1 (DOCK3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-4/5

Plasmid

#91265

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-4/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-4/5 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNMBP-1/6

Plasmid

#91276

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-1/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-1/6 (DNMBP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pACT2-Esf1

Plasmid

#99988

Purpose

Express yeast Esf1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Utp15

Plasmid

#99978

Purpose

Express yeast Utp15 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Utp15

Plasmid

#99977

Purpose

Express yeast Utp15 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
pHH0103_CASKIN2-1/1

Plasmid

#91261

Purpose

Protein expression and purification of human SH3 domain construct CASKIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CASKIN2-1/1 (CASKIN2 Human)

Expression

Bacterial

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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PatoK-OG241

Plasmid

#72840

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PatoM-OG241

Plasmid

#72842

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Expression

Bacterial

Promoter

PatoM

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PatoK-LOG241

Plasmid

#72848

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LPhP-pHG165

Plasmid

#90047

Purpose

Constitutive expression of a chimeric LacI:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LPhP

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega1R

Plasmid

#68240

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega2R

Plasmid

#68241

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-343

Plasmid

#61759

Purpose

TvoVMAdC48 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdC48 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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