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pET-SU(Z)12(624-900)-H6

Plasmid

#183772

Purpose

Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

Insert

SU(Z)12 residues 624-900 (Su(z)12 Fly)

Tags

His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.GFP.miR-ctrl

Plasmid

#169305

Purpose

Constitutive overexpression of miR-ctrl with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

EGFP

Promoter

SFFV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pWN10042

Plasmid

#89052

Purpose

GF-ori-FP plasmid for GFxFP assay

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

EGFP part 1
EGFP part 2

Use

CRISPR

Expression

Mammalian

Promoter

CAG and none

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Expression

Mammalian

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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B1-INTEGRIN WT

Plasmid

#195215

Purpose

Mammalian expression of mCherry tagged B1 Integrin. Wild-type.

Depositor

David Bryant, Valerie Weaver

Article

Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

Insert

ITGB1 (ITGB1 Human)

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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B1-INTEGRIN VN

Plasmid

#195216

Purpose

Mammalian expression of mCherry tagged B1 Integrin. Autoclustering mutant.

Depositor

David Bryant, Valerie Weaver

Article

Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

Insert

ITGB1 (ITGB1 Human)

Expression

Mammalian

Mutation

V737N

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKD

Plasmid

#86695

Purpose

Tetracycline inducible expression of shRNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-S2215Y

Plasmid

#69013

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2215Y

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO

Plasmid

#86696

Purpose

Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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