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His6-TEV-LplA W37V and A48N

Plasmid

#248823

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and A48N

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and L172A

Plasmid

#248815

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and L172A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V

Plasmid

#248814

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA

Plasmid

#248813

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplA Wild Type

Plasmid

#248801

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and L172A

Plasmid

#248803

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and L172A

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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VN1272 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-2GG+rpoA-IP3

Plasmid

#235120

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Type

Empty backbone

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

rpoA

Expression

Bacterial

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN519 pBR-rpoA-IP3mut3A

Plasmid

#235097

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses IP3mut3A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

IP3mut3A

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

rpoA

Expression

Bacterial

Promoter

lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN515 pBR-rpoA-IP1

Plasmid

#235098

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

rpoA

Expression

Bacterial

Promoter

lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN516 pBR-rpoA-IP2

Plasmid

#235099

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

rpoA

Expression

Bacterial

Promoter

lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN517 pBR-rpoA-IP3

Plasmid

#235100

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

rpoA

Expression

Bacterial

Promoter

lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN518 pBR-rpoA-IP4

Plasmid

#235101

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

rpoA

Expression

Bacterial

Promoter

lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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IAA17-4flag-HIS5

Plasmid

#207015

Purpose

For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHFdist_rm

Plasmid

#162318

Purpose

P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

IHF distal site is removed

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-DLDH-IRES-tdTomato

Plasmid

#178311

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1050Z4 (yellow)

Plasmid

#132425

Purpose

express arrays of gRNA targeting Yellow under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

yellow gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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