We narrowed to 3,167 results for: tef
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Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_MCART1
Plasmid#140591PurposeExpress MCART1 in S. cerevisiaeDepositorInsertSLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
ExpressionYeastMutationcodon-optimized for expression in S. cerevisiaePromoterNDT1 promoter and 3'UTRAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1464
Plasmid#111440PurposeN. castellii Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pH3F
Plasmid#85773PurposeN-ICE plasmid pH3FDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHB4
Plasmid#53462PurposeThis plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.DepositorInsertyeGFP-Snc1-Suc2
ExpressionYeastPromoterADHAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-GFPγ-SpHis5
Plasmid#60781PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selectionDepositorInsertGFPγ
UseYeast integratingMutationT65C S72A V163A I167T L231HAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
SIV URA PCP-NLS-GFPEnvy
Plasmid#189941PurposeSingle-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiaeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCP-NLS-GFPEnvy
ExpressionMammalianAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKar2-msGFP-HDEL
Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
ExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen (pKBJ010)
Plasmid#173461PurposeC-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-RFP-kanMX6
Plasmid#105160PurposeYeast gene targetingDepositorInsertGBP-RFP-kanMX6
TagsGBP-RFPExpressionBacterial and YeastAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIS3p:mEos2-Tub1+3'UTR::HPH
Plasmid#50634DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmEos2-Tub1
UseYeast expressionTagsmEos2PromoterHIS3pAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKT0232
Plasmid#8727DepositorPublicationInsertyECFP
Tags13x Myc and Codon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163AAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-PYK1
Plasmid#85777PurposeN-ICE plasmid pK3FS-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDH6
Plasmid#83777PurposeUseful for tagging yeast proteins with C-terminal YFP tag; G418 selectionDepositorPublicationInsertYFP
TagsYFPExpressionYeastMutationS65G, V68L, Q69K, S72A, T203Y mutations added.Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
THI VP64 pGAP T-
Plasmid#161487PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T1
Plasmid#161488PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T1 in the THI11 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T5
Plasmid#161492PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS303K-Pgal-OsTIR1(F74G)-GEM
Plasmid#247622PurposeExpression of OsTIR1DepositorInsertOsTIR1
ExpressionYeastMutationF74GPromoterGAL1Available sinceJan. 2, 2026AvailabilityAcademic Institutions and Nonprofits only