We narrowed to 19,741 results for: Cre

1. Pf92-bio

   Plasmid

   #47728

   Purpose

   Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised Pf92
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_RBMX-eA3A-UdgX-NG-nCas9

   Plasmid

   #163551

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

   Plasmid

   #170217

   Purpose

   Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CASP8 (CASP8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   C360A

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC8_PH-6His-Nter-GWs-Lox (VE5587)

   Plasmid

   #163768

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   N-terminal 6His tag
   

   Tags

   6 His Tag

   Expression

   Insect

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC8_MF-PH-10His-Cter (VE5631)

   Plasmid

   #161802

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal 10 His tag
   

   Tags

   10 His

   Expression

   Insect

   Promoter

   p10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

   Plasmid

   #139770

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal TwinStrep tag
   

   Tags

   3C - TwinStrep tag

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC8_MF-p10-10His-Cter (VE5742)

   Plasmid

   #139776

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal 10His tag
   

   Tags

   10 His

   Expression

   Insect

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC8_MF-p10-Flag-Cter(VE5738)

   Plasmid

   #161798

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal Flag tag
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   p10

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC8_PH-10His-Nter-GWs-Lox (VE5588)

   Plasmid

   #161806

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   N-terminal 10His tag
   

   Tags

   10 His

   Expression

   Insect

   Promoter

   PH

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC8_MF-p10-mCh PH-mCh (VE5625)

    Plasmid

    #139769

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    mCherry fluorescent protein
    

    Expression

    Insect

    Promoter

    PH or p10

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. GET1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176985

    Purpose

    Mammalian expression vector encoding GET1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GET1 (GET1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid

    #121813

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. KHBD00649

    Plasmid

    #39711

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6172 (vnd Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

    Plasmid

    #163138

    Purpose

    Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

    Plasmid

    #170225

    Purpose

    Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    ER (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    Y537S

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Islr-AP-His

    Plasmid

    #71950

    Purpose

    Expresses the entire Islr protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Islr (Islr Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST27-GST-ATXN1 FL(30Q)-S776D

    Plasmid

    #21755

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pECE-M2-BAIAP2 S366A

    Plasmid

    #31657

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 S366A (BAIAP2 Human)
    

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 366 to Alanine

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

19. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid

    #121800

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121819

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge