We narrowed to 17,264 results for: tes
-
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRExpressionMammalianPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorPublicationInsertkkv-R896K::NG (kkv Fly)
TagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-puro-3xFLAG-RUNX3 R122C
Plasmid#203439PurposeMammalian expression of RUNX4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRUNX3 (RUNX3 Human)
Tags3 x FLAGExpressionMammalianMutationR122CAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA
Plasmid#196896PurposeNeuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmidsDepositorAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-PSK2 (K57A)
Plasmid#197118PurposeExpression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cellsDepositorInsertTAOK1 (K57A) (TAOK1 Human)
TagsMycExpressionMammalianMutationChanged K 57 to APromoterSV40Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3763 : CMVd3-HsIRE1dLKR-HaloTag
Plasmid#185685PurposeLow-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 (ERN1 Human, Synthetic)
TagsHaloTagExpressionMammalianMutationDeletion of linker, kinase, and Rnase domainsAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
kanMX6-ins1
Plasmid#195038PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertKanR
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 899
Plasmid#194193PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseMutationmutated KLF binding sites (position 899)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut
Plasmid#187154Purposemammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHUWE1 (HUWE1 Human)
Tags1xFLAGExpressionMammalianMutationL2976A/P2977A/M1328A/F1330A/A1376G/S1382AAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A
Plasmid#182047Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationS248APromoterCAGAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x
Plasmid#182256PurposeHuman PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
UseLuciferaseExpressionMammalianAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
Plasmid#182049Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationS248A/T258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 T258A
Plasmid#182048Purposeexpression of Sox2 mutantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSox2 (Sox2 Mouse)
TagsFLAGExpressionMammalianMutationT258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Asp115Ala
Plasmid#180760PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)DepositorInsertα-COPI-WD40-Asp115Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Asp115Ala, Leu…PromoterCMVAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (B.1.351 lineage / South African variant)
Plasmid#169849PurposeMammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertProtein S
TagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMVtight-PLXNB2 iOE
Plasmid#176849Purposelentiviral vector for doxycycline-inducible PLXNB2 overexpressionDepositorAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPC-N-Myc-Flag-BirA-hPOT1
Plasmid#166409Purposeexpress BirA-hPOT1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthPOT1 (POT1 Human)
TagsBirA, flag, and mycExpressionMammalianPromotercmvAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-V12-puro
Plasmid#156168PurposeLentiviral vector for expression of flag-RAP1B-V12, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP-CoV2/RBD-puro
Plasmid#161793PurposeTransposon-based SARS-CoV-2 RBD stable expression vector for protein productionDepositorInsertSARS-CoV-2 RBD
UseTransposonTags6xHis and dTomatoExpressionMammalianPromoterEF1a/RPBSAAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by