161,152 results

1. HP17_KO7

   Plasmid

   #120346

   Purpose

   Helper plasmid for production of phagemid ssDNA, based on M13KO7

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Coding part of the M13KO7 helper phage genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   native phage promoters

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC-DPP4

   Plasmid

   #172416

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   Dipeptidyl peptidase 4 (DPP4 Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. PA-GFP-α-Tubulin

   Plasmid

   #213900

   Purpose

   Imaging of Tubulin using Photoactivatable GFP

   Depositor

   Lukas Kapitein

   Insert

   PA-GFP-α-Tubulin
   

   Use

   Tags

   Photoactivatable GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-No-HaloTag7-mGFP-LC3-mRFP

   Plasmid

   #184902

   Purpose

   Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7, monomeric EGFP, and monomeric RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MI-Luciferase-IRES-mCherry

   Plasmid

   #75020

   Purpose

   Encodes the expression of Luciferase and mCherry

   Depositor

   Xiaoping Sun

   Article

   Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

   Inserts

   firefly Luciferase
   mCherry
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1765 (pSPIN-R, R*MmeI)

   Plasmid

   #160734

   Purpose

   Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0987

   Plasmid

   #142715

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF2 (KLF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-FLAG

   Plasmid

   #60360

   Purpose

   Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp6-mNeongreen

   Plasmid

   #182388

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid

    #107738

    Purpose

    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

    Depositor

    Rylan Larsen

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre, dTomato
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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