170,823 results

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pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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GSK3betaS9A (1016)

Plasmid

#49492

Purpose

expresses human Gsk3beta with S9A (constitutively active) mutation

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta S9A (GSK3B Human)

Expression

Mammalian

Mutation

S9A (constitutively active)

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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hEGFR-mEGFP

Plasmid

#182878

Purpose

Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

Depositor

Salvatore Chiantia

Article

Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

Insert

hEGFR-mEGFP (EGFR Human)

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloCKAR2.2-T2A-EGFP

Plasmid

#216493

Purpose

Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloCKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s-CAAX

Plasmid

#52228

Purpose

Plasma membrane targeted GCaMP6s, calcium sensor

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

GCaMP6s-CAAX

Tags

6xHis and CAAX

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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5KO9 (HMCES)

Plasmid

#101877

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HMCES (HMCES Human)

Tags

6x His (modified C-term tag is AENLYFQSHHHHHH)

Expression

Bacterial

Promoter

T7

Available Since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

Plasmid

#198515

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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