We narrowed to 58,610 results for: %s

1. pcDNA3.1(-)-V5.hOr47a

   Plasmid

   #126474

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(-)-V5.mER.hOr47a

   Plasmid

   #126473

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Use

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDECKO_mCherry_Rat_cebp-alfa

   Plasmid

   #140098

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_mCherry_SPI1

   Plasmid

   #140099

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-hu-c-Myc-dN1

   Plasmid

   #50773

   Purpose

   retroviral expression of human c-Myc dN1 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN1 (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 14-439

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. shCTL-mlpx-puro

   Plasmid

   #65232

   Purpose

   encodes a non-targeting control shRNA

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   non-coding shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmirGLO-Bmpr2-Δ17

   Plasmid

   #49380

   Purpose

   Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.

   Depositor

   Martine Roussel

   Article

   Murphy et al Cancer Res. 2013 Oct 21.

   Insert

   Bmpr2 (BMPR2 Human, Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   changed nucleotides AC to TG at bp 7347-7348

   Promoter

   Human phosphoglycerate kinase (PGK) promoter

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcGFP1-N1-RvCAHS8-GS

   Plasmid

   #192461

   Purpose

   Expression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS8
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA pDEST40 Cas9 GW STOP

   Plasmid

   #183196

   Purpose

   Destination vector

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSET-A2-77p-Venus

    Plasmid

    #64856

    Purpose

    Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

    Depositor

    Sriram Neelamegham

    Article

    Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

    Insert

    VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
    

    Use

    Tags

    7X His and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLR5-CAG-NLS-tdiRFP670-2A-LBR

    Plasmid

    #82620

    Purpose

    Expresses NLS-tdiRFP-2A-LBR in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRShygro shID3#1

    Plasmid

    #19164

    Depositor

    Joan Massague

    Article

    Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

    Insert

    small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pET28_6xHis-PARP1-W318R

    Plasmid

    #174793

    Purpose

    Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-W318R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    W318, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV V5-nurim

    Plasmid

    #175110

    Purpose

    Lentiviral expression of V5-tagged mouse Nrm

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Nrm (Nrm Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3/flag-pBirA(1-G78)-FRB

    Plasmid

    #154447

    Purpose

    expresses pBirA(1-G78)-FRB/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pBirA(1-G78)-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET23a_mod_RBPMS_FL

    Plasmid

    #59393

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    6xHis, FLAG, and HA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221 Cas9-HMGB1

    Plasmid

    #183198

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-HMGB1
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. mKikGR-Tubulin-6

    Plasmid

    #57330

    Purpose

    Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mKikGR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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