We narrowed to 59,498 results for: %s

1. pLenti-PGK-EGFPc3-Neo

   Plasmid

   #189741

   Purpose

   The construct was used to express GFP in mammalian cells as the control for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX5X.1 PINK1 KD

   Plasmid

   #13322

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 kinase domain (PINK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 112-496; Kinase dead: K219D/D362A/D3…

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

3. TKTL1

   Plasmid

   #72419

   Purpose

   Study the role of aberrant expression of TKTL1 in HNSCC tumorigenesis

   Depositor

   David Sidransky

   Article

   Sun et al Clin Cancer Res. 2010 Feb 1;16(3):857-66

   Insert

   TKTL1 (TKTL1 Human)
   

   Expression

   Mammalian

   Mutation

   may have one or two synonymous substitutions

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. SspB-HaloTag-DHPH-ITSN1

   Plasmid

   #176113

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ePB-PURO-FLAG-METTL3-APPA

   Plasmid

   #160253

   Purpose

   For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   tight TRE promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-shSmad2

   Plasmid

   #37051

   Depositor

   Sam Thiagalingam

   Article

   Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

   Insert

   Smad2 (SMAD2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. px330 Rosa26 Gu1 (LH416)

   Plasmid

   #99629

   Purpose

   Plasmid used to target the wildtype Rosa26 allele with Cas9

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SI-112

   Plasmid

   #131127

   Purpose

   C-to-T base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Mutant EGFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-hPhyB621-mCherry-HRasCT

   Plasmid

   #100282

   Purpose

   Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-621 aa) (PHYB Mustard Weed)
   

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-621 aa)

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FpG5.BFP.Reporter

    Plasmid

    #134967

    Purpose

    Lentiviral vector for enhancer reporter assay

    Depositor

    Beat Bornhauser

    Article

    Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mini Fgf4 promoter

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Mito-AcGFP-MTn

    Plasmid

    #154350

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Tags

    AcGFP

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

    Plasmid

    #182231

    Purpose

    Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Inserts

    ras homolog family member B (RHOB Human)
    rhotekin RHO binding domain (RBD)
    

    Use

    Lentiviral

    Tags

    GFP10 (split-GFP tripartite) and GFP11 (M4)

    Promoter

    TRE bidirectional

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS1859

    Plasmid

    #205972

    Purpose

    FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS1860

    Plasmid

    #205973

    Purpose

    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-HE-Glyco-Venus-bPAC(S27A)

    Plasmid

    #165490

    Purpose

    Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Glyco-Venus-bPAC(S27A)-Myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-FCHO1

    Plasmid

    #196910

    Purpose

    visualization of clathrin-coaed vesicle formation in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

    Insert

    FCHO1 (FCHO1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-V5-Lem2

    Plasmid

    #120245

    Purpose

    Expresses V5-tagged Lem2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Lem2 (Lemd2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Arrb1-Q394L

    Plasmid

    #35402

    Depositor

    Robert Lefkowitz

    Article

    Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

    Insert

    beta-arrestin-1 (Arrb1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q394L

    Promoter

    CMV

    Available Since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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