We narrowed to 12,204 results for: 112

1. pRK5-myc Metap2

   Plasmid

   #199685

   Purpose

   Transient expression of Metap2

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   METAP2 (METAP2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

   Plasmid

   #198840

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ATP8-W9-L17 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

   Plasmid

   #198844

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX2-Q6-L18 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-delLN-DmPABPC1-V5His6_H

   Plasmid

   #146477

   Purpose

   Insect Expression of DmPABPC1

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmPABPC1 (pAbp Fly)
   

   Expression

   Insect

   Mutation

   one non silent mutation G26S, and seven silent mu…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

   Plasmid

   #193719

   Purpose

   Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Synthetic, Human)
   

   Tags

   2xHA-FRB

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

   Plasmid

   #194894

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_K365A_R368A
   U6-crRNA2
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

   Plasmid

   #194886

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_K365A_R368A
   U6-crRNA1and2
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_Tau Authentic Alu 10-12K317M

   Plasmid

   #194168

   Purpose

   Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 K317M
   

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Lysine 317 to Methionine

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

   Plasmid

   #191331

   Purpose

   Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
   

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-GG-hUbC-EBPF2-gRNA-RBMS3

    Plasmid

    #185557

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    RBMS3 gRNAs
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-GG-hUbC-EBPF2-gRNA-HHIP

    Plasmid

    #185551

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting HHIP

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    HHIP gRNAs
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-GG-hUbC-EBPF2-gRNA-ADGRG6

    Plasmid

    #185554

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    ADGRG6 gRNAs
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXPR_BRD003 FDX1-1

    Plasmid

    #184476

    Purpose

    Lentivirus gRNA targeting human FDX1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR_BRD003 LIPT1-2

    Plasmid

    #184481

    Purpose

    Lentivirus gRNA targeting human LIPT1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT1 (LIPT1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_BRD003 LIPT1-1

    Plasmid

    #184480

    Purpose

    Lentivirus gRNA targeting human LIPT1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT1 (LIPT1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXPR_BRD003 LIPT2-2

    Plasmid

    #184483

    Purpose

    Lentivirus gRNA targeting human LIPT2 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT2 (LIPT2 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXPR_BRD003 FDX1-2

    Plasmid

    #184477

    Purpose

    Lentivirus gRNA targeting human FDX1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_BRD003 LIPT2-1

    Plasmid

    #184482

    Purpose

    Lentivirus gRNA targeting human LIPT2 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT2 (LIPT2 Human)
    

    Use

    Lentiviral

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmxs_COX4

    Plasmid

    #177979

    Purpose

    Mammalian expression of COX4 cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    COX4 (COX4I1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Silent point mutations to COX4 sgRNA target

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE30-VRN1-R249E-R289E-R296E

    Plasmid

    #159532

    Purpose

    Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

    Depositor

    Joshua Mylne

    Article

    King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

    Insert

    VERNALIZATION1 (VRN1 Mustard Weed)
    

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Three charge reversal mutations, namely R249E R28…

    Promoter

    T5

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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