We narrowed to 17,098 results for: IGH@

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pIGc-Xph18

Plasmid

#133029

Purpose

Expresses Xph18 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph18

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph15

Plasmid

#133030

Purpose

Expresses Xph15 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph15

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

Plasmid

#72889

Purpose

fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO2) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

Inserts

Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
Volvox channelrhodopsin 1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA105

Plasmid

#128521

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme T70N (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-TET1-746-951(pL72)

Plasmid

#124406

Purpose

TET1-746-951, containing SID, used for Co-IP.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-TET1-746-951 (TET1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

Plasmid

#114373

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv4.2LL (NEWENTRY )

Use

AAV

Tags

EYFP and Kv4.2LL

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

Plasmid

#114375

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

Plasmid

#114372

Purpose

expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Nlgn1C (NEWENTRY )

Use

AAV

Tags

EYFP and Nlgn1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)

Plasmid

#112803

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Jurkiewicz et al Biochim Biophys Acta. 2017 May;1864(5):7

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 554_557 deleted/PDZ

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DEPTOR_DEP

Plasmid

#109882

Purpose

Protein expression and purification of DEPTOR_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DEPTOR_DEP (DEPTOR Human)

Expression

Bacterial

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTG-mTDGa.0

Plasmid

#81048

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Tags

6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-N-SH2

Plasmid

#111274

Purpose

express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)

Tags

HHHHHHENLYFQG

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMflCT-o4

Plasmid

#101313

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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