Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,255 results for: TIM

Clear keywords
Showing: 11441 - 11460 of 14255 results

  1. pAAV-EF1a-N-CretrcintG

    Plasmid
    #69570
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - N-CretrcintG
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69570-plasmid-map-sequence-id-127742

    Enlarge

  2. pAAV-EF1a-C-CreintG

    Plasmid
    #69571
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - C-CreintG
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69571-plasmid-map-sequence-id-127747

    Enlarge

  3. QQR1 variant

    Plasmid
    #138564
    Purpose
    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    QQR1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138564-plasmid-map-sequence-id-274793

    Enlarge

  4. phage UbiC scAB-GFP

    Plasmid
    #104998
    Purpose
    Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    single chain Antibody against GCN4
    Use
    Lentiviral
    Tags
    GB1, HA tag, and sfGFP
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    104998-plasmid-map-sequence-id-202897

    Enlarge

  5. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138566-plasmid-map-sequence-id-274798

    Enlarge

  10. pHUE411

    Plasmid
    #62203
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62203-plasmid-map-sequence-id-467234

    Enlarge

  17. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

    Enlarge

Showing: 11441 - 11460 of 14255 results