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pBEG R3-miRNA(ccdB)-L4 #BGV228

Plasmid

#48953

Purpose

To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Promoter

na

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
Clover-PDHA1-N-10

Plasmid

#56304

Purpose

Localization: Mitochondria, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PDHA1 (PDHA1 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGEL033

Plasmid

#137902

Purpose

STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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shNTC

Plasmid

#73549

Purpose

Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shNTC

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pOCC28

Plasmid

#118873

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease and MBP, cleavab…

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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PU.1-ets-IRES-mCherry

Plasmid

#80141

Purpose

retroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

PU.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

ets = PU.1 DNA binding domain (amino acids 159-26…

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pOCC291

Plasmid

#118867

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease, mRuby3

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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peCALWY-2

Plasmid

#22234

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-2 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

sequence identical to eCALWY-1, but linkerlength …

Available Since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
pRmHA3-CH2F2-CG9866

Plasmid

#52286

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Tags

2xHA 2xFLAG

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pGEL029

Plasmid

#137898

Purpose

STU2.0 SpyCas9 RZ system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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