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pAc5.1-lambdaN-HA-DmDCP1

Plasmid

#21685

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC276

Plasmid

#19637

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC245

Plasmid

#19638

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R4-R3)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC246

Plasmid

#19673

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

RNAi; Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC193

Plasmid

#19686

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

hlh-12(R25K)

Use

Gateway Cloning

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pGC114

Plasmid

#19622

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC163

Plasmid

#19635

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC305

Plasmid

#19646

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-1xNLS-GFP::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC307

Plasmid

#19662

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-1xNLS-CFP::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC381

Plasmid

#19663

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*-CFP::let-858(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-C67Y

Plasmid

#10982

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C67Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 200 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C70Y

Plasmid

#10983

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C70Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 209 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-H80Y

Plasmid

#10984

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-H80Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 238 C to T in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pRB326

Plasmid

#8667

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Use

Centromeric

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pIRES LifeAct-GFP neo3

Plasmid

#64356

Purpose

Bicistronic expression of LifeAct-GFP fusion and neo3 resistance gene

Depositor

Matthieu Piel

Insert

LifeAct-GFP

Tags

IRES-Neo

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-sgLkb1/Cre

Plasmid

#66894

Purpose

Expresses an Lkb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgLkb1

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pPro18-cm

Plasmid

#17807

Depositor

Jay Keasling

Article

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
pLenti-SFNp-mRFP1

Plasmid

#238473

Purpose

Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone

Depositor

Christopher Nelson

Article

Ivy et al BMC Methods. 2025;2(1):8. doi: 10.1186/s

Insert

SFNp-mRFP1

Promoter

SFNp

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MCP-EGFP-PiggyBac

Plasmid

#190003

Purpose

Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-EGFP

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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CAP-AAV.FUS.3

Plasmid

#222333

Purpose

AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

Depositor

Mikhail Shapiro, Jerzy Szablowski

Article

Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

Insert

AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1

Use

AAV

Promoter

p41

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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