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actc1b-BTK-mKate2

Plasmid

#109501

Purpose

Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-BTK-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CaaX(tH)

Plasmid

#109487

Purpose

Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-CaaX(tH)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CaaX(tK)

Plasmid

#109488

Purpose

Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-CaaX(tK)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-HypaCAS9-pA

Plasmid

#131467

Purpose

Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

Insert

Codon optimized HypaCas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBW012

Plasmid

#131815

Purpose

Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55

Depositor

Jonathan Silberg

Article

Wu et al bioRxiv May 23, 2019

Insert

M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55

Use

Synthetic Biology

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseC

Plasmid

#89724

Purpose

Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseC (pseC Campylobacter jejuni)

Tags

His8 and TEV

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only
pZA-mCherry-R5

Plasmid

#106258

Purpose

Expresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetO

Depositor

Urartu Seker

Article

Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

Insert

mCherry

Tags

Added silaffin R5 peptide to C terminus of mCherr…

Expression

Bacterial

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only
pPKm-195

Plasmid

#90496

Purpose

pcDNA3 - CMVmin - PhyB - MTAD, expresses PhyB and MTAD, under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PhyB NT-MTAD

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK1-1/1-SV1/3

Plasmid

#91548

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV1/3 (TNK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP4-1/1

Plasmid

#91518

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP4-1/1 (ARHGAP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3GL1-1/1

Plasmid

#91473

Purpose

Protein expression and purification of human SH3 domain construct SH3GL1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GL1-1/1 (SH3GL1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SLA2-1/1

Plasmid

#91450

Purpose

Protein expression and purification of human SH3 domain construct SLA2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SLA2-1/1 (SLA2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP9-1/1

Plasmid

#91442

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP9-1/1 (ARHGAP9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DBNL-1/1

Plasmid

#91409

Purpose

Protein expression and purification of human SH3 domain construct DBNL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DBNL-1/1 (DBNL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NEBL-1/1

Plasmid

#91383

Purpose

Protein expression and purification of human SH3 domain construct NEBL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NEBL-1/1 (NEBL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF16-1/1

Plasmid

#91334

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF16-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF16-1/1 (ARHGEF16 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPTAN1-1/1

Plasmid

#91292

Purpose

Protein expression and purification of human SH3 domain construct SPTAN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SPTAN1-1/1 (SPTAN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AHI1-1/1

Plasmid

#91269

Purpose

Protein expression and purification of human SH3 domain construct AHI1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AHI1-1/1 (AHI1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Expression

Bacterial

Promoter

T7/M13

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-004

Plasmid

#78691

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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