We narrowed to 746 results for: PIP

1. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Use

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-myc-BirA*-NRASG12V

   Plasmid

   #122209

   Purpose

   Expresses myc-BirA* fused to NRASG12V in mammalian cells

   Depositor

   Christopher Counter

   Article

   Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

   Insert

   NRASG12v (NRAS Human)
   

   Use

   Tags

   myc-BirA*

   Expression

   Mammalian

   Mutation

   changed G12 to V

   Promoter

   CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST40-2XHA-Src-S4-UD-S32

   Plasmid

   #140302

   Purpose

   Expresses HA-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   lacks the KD region

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST40-2XHA-Src-S4-S32-KD

   Plasmid

   #140298

   Purpose

   Expresses HA-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   lacks the UD region

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-MmCENP-O-GFP

   Plasmid

   #205178

   Purpose

   Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

   Insert

   CENP-O (Cenpo Mouse)
   

   Use

   In vitro transcription

   Tags

   5 glycines linker followed by GFP

   Expression

   Mammalian

   Mutation

   Wild type mouse CENP-O

   Promoter

   T7

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB_EA_R1CiM_4EGFP_R3EGFPi2

   Plasmid

   #185968

   Purpose

   Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Molecular IMPLY gate
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB_EA_R3tdTomatoi2

   Plasmid

   #185969

   Purpose

   Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PR

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST40-2XHA-Src-Y530EEI

   Plasmid

   #140322

   Purpose

   Expresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Y530EEI

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-2XFL-Src-S4-UD

   Plasmid

   #140305

   Purpose

   Expresses Flag-tagged human Src-S4-UD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   contains only the SH4 and UD regions

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST40-2XFL-Src-S4-UD-KD

    Plasmid

    #140301

    Purpose

    Expresses Flag-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    lacks the SH3 and SH2 domains

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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