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Plasmid#234429PurposePlasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified dCas9(D10A, H840A) with polyaspartate linking
UseTagsExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH2 #7
Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleInsertIDH2 sgRNA #7 (IDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleInsertIDH1 sgRNA #4 (IDH1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-P2A-mCherry BlastR
Plasmid#186743PurposeDox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertP2A-mcherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGIPZ-PD-L1-EGFP
Plasmid#120933PurposeExpress PD-L1-EGFP fusion protein in mammalian cellsDepositorInsertPD-L1-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-OTX2-P2A-mCherry BlastR
Plasmid#186741PurposeDox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-shRab27a
Plasmid#120930Purposeconditionally knockdown Rab27a in mouse cell linesDepositorInsertshRab27a (Rab27a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-C1-hSERT
Plasmid#70103Purposemammalian expression of hSERT with N-terminal YFP fusionDepositorInsertSERT (SLC6A4 Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pECFP-C1-hSERT
Plasmid#70104Purposemammalian expression of hSERT with N-terminal CFP fusionDepositorInsertSERT (SLC6A4 Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR
Plasmid#186745PurposedCas9-10XGcn4-P2A-mCherry expression constructDepositorInsertCas9-10XGcn4-P2A-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only