We narrowed to 617 results for: spen

1. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

   Plasmid

   #113158

   Purpose

   An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

   Plasmid

   #173963

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   1 mutated Coordinator motif

   Promoter

   SV40 promoter

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

   Plasmid

   #113162

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGG369

   Plasmid

   #165606

   Purpose

   Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

   Expression

   Bacterial

   Mutation

   Recoding of BsaI site in the AmpR cassette, two B…

   Promoter

   lacUV2 driving ω-dCas9 and UV5 driving sgRNA

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

   Plasmid

   #113156

   Purpose

   An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat Rosa26
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#2

   Plasmid

   #173986

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #2 mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Coordinator motif mutation #2

   Promoter

   SV40 promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-noSV40-EC1.45-human_min1-opossum_min2

   Plasmid

   #173966

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   SV40 promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

   Plasmid

   #173994

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   7X Coordinator motif mutation #1-2-3-4-5-6-7

   Promoter

   SV40 promoter

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

   Plasmid

   #173962

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   4 mutated Coordinator motifs

   Promoter

   SV40 promoter

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#1

    Plasmid

    #173985

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Coordinator motif mutation #1

    Promoter

    SV40 promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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