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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_CyOFP1_KCNQ2 var4

Plasmid

#173155

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-CyOFP1

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) L533E V5 His

Plasmid

#119288

Purpose

CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

L533E

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-ArchT-GFP

Plasmid

#58851

Purpose

AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) R522D V5 His

Plasmid

#119289

Purpose

CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

R522D

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)

Use

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85008

Purpose

For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85006

Purpose

For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-HT-LC3-SV40-Hygro

Plasmid

#182045

Purpose

Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

Depositor

Hong-Gang Wang

Article

Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

HaloTag-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Jaws-KGC-GFP-ER2

Plasmid

#65015

Purpose

AAV-mediated expression of Jaws under the CaMKII promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1P-iCLHN

Plasmid

#66579

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pC13P-iCLHN

Plasmid

#66580

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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fck-Jaws-GFP-ER2

Plasmid

#65013

Purpose

Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

Lentiviral

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FDIO-ArchT-GFP

Plasmid

#124640

Purpose

AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

ArchT

Use

AAV; Flp/frt

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-GFP-ER2

Plasmid

#65016

Purpose

AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

AAV

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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fck-Jaws-KGC-GFP-ER2

Plasmid

#65012

Purpose

Lentivirus-mediated expression of Jaws under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

Lentiviral

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET51b-Strep-hAGT-His

Plasmid

#167276

Purpose

Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

hAGT (AGT Human)

Use

Tags

His x10 and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#69138

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

Plasmid

#47904

Purpose

An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

eNpHR3.0

Use

AAV

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-KGC-GFP-ER2

Plasmid

#65014

Purpose

AAV-mediated expression of Jaws under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV Retrograde, AAV5, and AAV8

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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