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pTALETF_v2 (NG)

Plasmid

#32186

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NG for 0.5 repeat

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pTALETF_v2 (NI)

Plasmid

#32187

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NI for 0.5 repeat

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

Plasmid

#196844

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

Plasmid

#196847

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

Plasmid

#196846

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

Plasmid

#196845

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1R TALEN

Plasmid

#35432

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1R TALEN

Expression

Mammalian

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pMOLUC

Plasmid

#12514

Depositor

Tong-Chuan He

Article

Feng et al Biotechniques. 2002 May . 32(5):992, 994

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pmax-PUFc_NES-ADARdd(E488Q)

Plasmid

#196837

Purpose

Express PUFc_NES-ADARdd(E488Q)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

PUFc_NES-ADARdd(E488Q)

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEERM4 Cm

Plasmid

#64026

Purpose

Plasmid for integration into neutral site 2 of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEERM3 Km

Plasmid

#64025

Purpose

Plasmid for integration into neutral site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PpsbA2

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEERM6 Km

Plasmid

#64027

Purpose

Plasmid for integration into sqs site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pZZMP09

Plasmid

#231754

Purpose

Mammalian expression of SPO11

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11 (Spo11 Mouse)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Aa-artsg13 (BsaI)

Plasmid

#188279

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Aa-artsg13

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmax-MCP_NES-ADARdd(E488Q)

Plasmid

#196840

Purpose

Express MCP_NES-ADARdd(E488Q)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

MCP_NES-ADARdd(E488Q)

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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EF.PGK.GFP

Plasmid

#17618

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Tags

6xHis

Expression

Bacterial

Promoter

T5 promoter

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pZZMP122

Plasmid

#231756

Purpose

Mammalian expression of SPO11-YF

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-YF (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Y138F

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES

Plasmid

#196831

Purpose

Express PUFc-RESCUE-S

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

PUFc-RESCUE

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-tRNAx2

Plasmid

#123641

Purpose

Mammalian expression, Genome editing, backbone

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOG2003

Plasmid

#49516

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI RR in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Tags

FokI-RR, HA, and SV40NLS

Expression

Worm

Promoter

hsp-16.2

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pOG2002

Plasmid

#49514

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Tags

3xFLAG, FokI-AS, and SV40 NLS

Expression

Worm

Promoter

hsp-16.2

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pZZMP132

Plasmid

#231757

Purpose

Mammalian expression of SPO11-EA

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-EA (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

E224A

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pmax-hcoRBFOX1N-PUFc-RBFOX1C

Plasmid

#196830

Purpose

Express hcoRBFOX1N-PUFc-RBFOX1C(190-397)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

hcoRBFOX1N-PUFc-RBFOX1C(190-397)

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmax-hcoRBFOX1N-PCP-RBFOX1C

Plasmid

#196838

Purpose

Express hcoRBFOX1N-PCP-RBFOX1C(190-397)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

hcoRBFOX1N-PCP-RBFOX1C(190-397)

Tags

V5

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmax-hcoRBFOX1N-MCP-RBFOX1C

Plasmid

#196839

Purpose

Express hcoRBFOX1N-MCP-RBFOX1C(190-397)

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

hcoRBFOX1N-MCP-RBFOX1C(190-397)

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-Bs3-sgRNA (BsaI)

Plasmid

#188280

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Bs3-sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge