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We narrowed to 5,865 results for: pcDNA 3.1

Showing: 1711 - 1740 of 5865 results

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pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

Plasmid

#177773

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

K592A

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-G120E_W

Plasmid

#147819

Purpose

Mammalian Expression of HsSmg5-G120E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

Plasmid

#147558

Purpose

Mammalian Expression of HsSmg7_633-1091

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_633-1091 (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation V854I compared to the seq…

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD2

Plasmid

#180602

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-TRBP-dsRBD1

Plasmid

#180601

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Arg57Ala

Plasmid

#180759

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Arg57Ala

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Arg57Ala, Leu1…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Tyr139Ala

Plasmid

#180761

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Tyr139Ala

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Tyr139Ala, Leu…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Asp115Ala

Plasmid

#180760

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Asp115Ala

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Asp115Ala, Leu…

Promoter

CMV

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRBP-L2+D2+L3-myc/His

Plasmid

#180600

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer-stm2585

Plasmid

#174395

Purpose

Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain and sarA chimera

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585-gp130

Plasmid

#174393

Purpose

Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Use

Tags

Flag

Expression

Mammalian

Mutation

140-177 of sarA replaced with homologous IL6ST se…

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-PRGRH-Nluc

Plasmid

#158608

Purpose

Plasmid expressing PRG(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-p115RH-Nluc

Plasmid

#158607

Purpose

Plasmid expressing p115(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 mitoAURKA Lys162Met

Plasmid

#157752

Purpose

Expression of kinase-dead AURKA K62M in mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AURKA deltaNter

Plasmid

#157749

Purpose

Expression of AURKA without Nter in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

Expression

Mammalian

Mutation

the first 30AA of Aurora A are lacking

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DW164AA 1-205 USP7

Plasmid

#131254

Purpose

Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Expresses a mutant form of our pcDNA3.1-N-Myc_1-2…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575_AG

Plasmid

#148752

Purpose

Mammalian Expression of HsNot4_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_1-274_AG

Plasmid

#148750

Purpose

Mammalian Expression of HsNot4_1-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_1-274 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-33/myc-His

Plasmid

#127492

Purpose

Expresses myc-tagged _MTS NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-33 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-33

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-78/myc-His

Plasmid

#127493

Purpose

Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-78 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-78

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG

Plasmid

#127323

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG

Plasmid

#127305

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG

Plasmid

#127306

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AGG-nLuc-3XFLAG

Plasmid

#127304

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG

Plasmid

#127303

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GGG-nLuc-3XFLAG

Plasmid

#127307

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-deltaA1-R1597W-VWF

Plasmid

#124826

Purpose

Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and R1597W mutation A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-D1498A-VWF

Plasmid

#124825

Purpose

Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and D1498A mutation A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nod-BFP2-TSERex

Plasmid

#124785

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Nod-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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ELK1 AB box pcDNA3.1/HisC

Plasmid

#121094

Purpose

Expression of the AB box fragment of ELK1

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Use

Tags

6xHis-omni-express

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1711 - 1740 of 5865 results

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