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Plasmid#177101PurposeExpresses Aur1-msGFP2 in yeast cellsDepositorInsertAUR1 (AUR1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RBD2-6xmGFP
Plasmid#177109PurposeExpresses Rbd2-6xmEGFP in yeast cellsDepositorInsertRBD2 (RBD2 Budding Yeast)
UseTags6xmEGFPExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC2-msGFPx3
Plasmid#177110PurposeExpresses Sec2-msGFPx3 in yeast cellsDepositorInsertSEC2 (SEC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTAR2
Plasmid#166940PurposeSTAR-based multi-level gene expression controller (MLC)DepositorInsertCircuit expressing GFP- Small transcription activating RNAs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTAR
Plasmid#166939PurposeSTAR-based multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Small Transcription activating RNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
Plasmid#165029PurposeExpress cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherryDepositorInsertLaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)
Plasmid#165026PurposeExpress cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)
Plasmid#165028PurposeExpress cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K
Plasmid#112652PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K
UseTagsExpressionYeastMutationR59K (GAP-dead)PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K
Plasmid#112647PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K
UseTagsExpressionYeastMutationR54KPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV47
Plasmid#128866PurposePosition 1 module- IX301997 (yeast integrative region IX301997)DepositorInsertYeast integrative region chrIX301997
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV46
Plasmid#128865PurposePosition 1 module- VI260998 (yeast integrative region VI260998)DepositorInsertYeast integrative region chrVI260998
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0114
Plasmid#117553PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamianaDepositorInsertPromoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana
UseTagsExpressionPlantMutationPromoterAt U6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0029
Plasmid#117541PurposeLevel1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (LbCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0028
Plasmid#117540PurposeLevel1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (AsCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0014
Plasmid#117524PurposeLevel0 Golden Gate part: CDS, SpCas9-KADepositorInsertSpCas9-KA (with stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0009
Plasmid#117522PurposeLevel0 Golden Gate part: CDS, SpCas9-p (no stop codon)DepositorInsertSpCas9-p (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL90005
Plasmid#117520PurposeLevel0 Golden Gate part: CDS, SpCas9-h (no stop codon)DepositorInsertSpCas9-h (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0008
Plasmid#117535PurposeLevel0 Golden Gate part: CDS, AsCas12aDepositorInsertAsCas12a (with stop codon)
UseCRISPRTags3xHA and NLSExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbI S178A
Plasmid#114159PurposeExpresses ClbI S178ADepositorInsertclbI
UseTags6xHis and 6xHis-Thrombin siteExpressionBacterialMutationMutated S178 to AlaPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
miR766 in pcDNA3.1
Plasmid#78124PurposeMammalian expression of miR766DepositorInsertmiR766 (MIR766 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2336
Plasmid#67541PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS69
Plasmid#48367PurposeFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsertFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
FR_GFP
Plasmid#31302DepositorInsertgreen fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
CaMCLC-His
Plasmid#111292Purposebacterial expressionDepositorInsert(S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase
UseTagsHis tag, GGGSGGGSLEHHHHHHExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
MsMUT_sgRNA1
Plasmid#111296PurposeCRISPR KO murine MUTDepositorInsertsgRNA1 against murine MUT
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
MsMUT_sgRNA3
Plasmid#111297PurposeCRISPR KO murine MUTDepositorInsertsgRNA3 against murine MUT
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4G
Plasmid#104502PurposeUsed to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter geneDepositorInsertScPGM2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4K
Plasmid#104506PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSpGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4O
Plasmid#104510PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSpdGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4P
Plasmid#104511PurposeUsed to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSptGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Myosin-IIA-GFP
Plasmid#38297DepositorInsertMyosin II A (Myh9 Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
WFS1-Flag-pcDNA3
Plasmid#13011DepositorInsertWolfram Syndrome 1 (WFS1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
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ABE8r
Plasmid#208294PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP
Plasmid#45574PurposeExpresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backboneDepositorInsertco I-Sce I
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT PINK1 C-myc
Plasmid#13314DepositorInsertPINK1 (PINK1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGFP FAK
Plasmid#50515PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cag.Luc.puro
Plasmid#74409PurposeConstitutive Luciferase expression in mammalian cells along with puromycin selectionDepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30b_OmpAT7Sav
Plasmid#138589PurposeT7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
UseSynthetic BiologyTagsOmpA export signal and T7 tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT PINK1 N-myc
Plasmid#13313DepositorInsertPINK1 (PINK1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-StagRFP-EKAR-EV
Plasmid#173019PurposeRed/far-red FRET-based ERK activity reporter for multiplexed imaging applications.DepositorInsertsmURFP-StagRFP-EKAR-EV
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT277
Plasmid#122608Purposeexpress evoCDA1-BE4max in mammalian cellsDepositorInsertevoCDA1-BE4max
UseTagsExpressionMammalianMutationF23S A123V I195FPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PE-P3-rice
Plasmid#185612PurposeFor plant prime editing in rice plantsDepositorInsert3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-MCS
Plasmid#145245PurposeBackbone vector expressing GAL4 DNA-binding domain followed by MCSDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEZ-1
Plasmid#51868PurposeExpression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegansDepositorInsertpsdhb-1::mtLS-cpYFP
UseTagsExpressionWormMutationPromotersdhb-1Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-wildtype
Plasmid#179684PurposeExpression of base editor in mammalian cellsDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only