We narrowed to 5,868 results for: arl

1. pPBbsr2-Necl-5-ScNeo

   Plasmid

   #170283

   Purpose

   To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)
   

   Tags

   mScarlet and mNeonGreen

   Expression

   Mammalian

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. C99m

   Plasmid

   #121003

   Purpose

   MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. rk16s

   Plasmid

   #117436

   Purpose

   Expression of ARL4

   Depositor

   Richard Kahn

   Insert

   ARL4 (ARL4A Human)
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TC9pAB

   Plasmid

   #71310

   Purpose

   used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9
   

   Use

   In vitro transcription

   Tags

   NLS

   Promoter

   T7

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. C108m

   Plasmid

   #121010

   Purpose

   MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C103m

   Plasmid

   #121007

   Purpose

   MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized with LAA tag
   

   Use

   Synthetic Biology

   Tags

   LAA degradation tag for ssrA

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. rk18s

   Plasmid

   #117438

   Purpose

   Expression of ARL11

   Depositor

   Richard Kahn

   Insert

   ARL11 (ARL11 Human)
   

   Expression

   Bacterial

   Mutation

   C148R (see comments below)

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TC9nickpAB

   Plasmid

   #71311

   Purpose

   used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9 nickase
   

   Use

   In vitro transcription

   Tags

   NLS

   Mutation

   D10A nickase

   Promoter

   T7

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWXC078

   Plasmid

   #222961

   Purpose

   C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet-I3
   

   Expression

   Worm

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWXC077

    Plasmid

    #222960

    Purpose

    C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet3
    

    Expression

    Worm

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKSII/INSeGFP

    Plasmid

    #53787

    Purpose

    1 kb zebrafish insulin promoter driving eGFP

    Depositor

    Larry Moss

    Article

    diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

    Insert

    INS promoter/eGFP/pA
    

    Use

    Zebrafish

    Tags

    eGFP

    Expression

    Bacterial

    Promoter

    zebrafish insulin/ZIP

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET21b FAST-PETase

    Plasmid

    #225760

    Purpose

    Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    FAST-PETase
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21b Hot-PETase

    Plasmid

    #225761

    Purpose

    Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    Hot-PETase
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. cp196V1.2

    Plasmid

    #52100

    Purpose

    A improved cpmCherry variant

    Depositor

    Robert Campbell

    Article

    Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

    Insert

    cp196V1.2
    

    Tags

    6 His tag

    Expression

    Bacterial

    Promoter

    pBAD promotor

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. mRFP-Rab5

    Plasmid

    #14437

    Purpose

    Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 5 (RAB5A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a isPETase

    Plasmid

    #225759

    Purpose

    Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    isPETase
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJG103

    Plasmid

    #158533

    Purpose

    wrmScarlet11 x3 tandem repeats

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet11 x3 tandem repeats
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC-GoldyTALEN

    Plasmid

    #38143

    Purpose

    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

    Depositor

    Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Tags

    AcV5 and FokI homodimer

    Expression

    Mammalian

    Mutation

    Truncate at N and C terminous

    Promoter

    miniCaggs

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Stephen Ekker, Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmEGFP-N1 SYS1

    Plasmid

    #175092

    Purpose

    SYS1-mEGFP can be transfected in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Ishida et al J Cell Biol. 2019 Nov 4;218(11):3681-369

    Insert

    SYS1 (SYS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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