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pSFV_rTetR-VP16 (3'UTR)_RLEloop

Plasmid

#240257

Purpose

Attenuated PROTEUS vector with rtTA-3G transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

rTetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_TetR-VP16 (3'UTR)_RLEloop

Plasmid

#240259

Purpose

Attenuated PROTEUS vector with tTA transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

TetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pGTM_SUMO_PLpro_W106F_001

Plasmid

#234491

Purpose

Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a W106F mutation to allow for 5-fluorotryptophan incorporation and 19F NMR

Depositor

Gaetano Montelione

Article

De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do

Insert

PLpro (ORF1ab SARS-CoV-2)

Tags

6xHis-Sumo

Expression

Bacterial

Promoter

T7 ac

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CD86-Rho-tag

Plasmid

#249669

Purpose

Expression of monomeric rhodamine-binding protein tag on the cell surface in mammalian cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

CD86-Rho-tag

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn1-iCre-P2A-mRuby3

Plasmid

#235248

Purpose

AAV-mediated expression of iCre and mRuby3 in mammalian cells

Depositor

Takeshi Imai

Article

Egashira et al Sci Adv. 2026 Jan 16;12(3):eadw8458. doi

Inserts

iCre
mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn1

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-jGCaMP8m-P2A-CyRFP1-WPRE

Plasmid

#235249

Purpose

AAV-mediated and Cre-dependent expression of jGCaMP8m and CyRFP1 in mammalian cells

Depositor

Takeshi Imai

Article

Egashira et al Sci Adv. 2026 Jan 16;12(3):eadw8458. doi

Inserts

jGCaMP8m
CyRFP1

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pGuide-RUNX3

Plasmid

#246659

Purpose

Guide RNA for human RUNX3

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

RUNX3 guide RNA (RUNX3 Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E53TAG)-mCherry

Plasmid

#241293

Purpose

Mammalian expression of syntaxin-1(E53TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E206TAG)-mCherry

Plasmid

#241295

Purpose

Mammalian expression of syntaxin-1(E206TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E206TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K84TAG)-mCherry

Plasmid

#241297

Purpose

Mammalian expression of syntaxin-1(K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E49TAG)-mCherry

Plasmid

#241291

Purpose

Mammalian expression of syntaxin-1(E49TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mIRFP670nano3

Plasmid

#244107

Purpose

Bacterial expression of green glucose sensor with inert mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Tags

mIRFP670nano3

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mStayGold

Plasmid

#234549

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mStayGold(Ando) fusion protein

Depositor

Hajime Hirase

Article

Knak et al Neurophotonics. 2025 Jun;12(Suppl 2):S22

Insert

Alb-mStayGold

Use

AAV

Mutation

None

Promoter

P3

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SQLE_sg2

Plasmid

#244872

Purpose

Knockout of human SQLE

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SQLE sgRNA (SQLE Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SQLE_sg1

Plasmid

#244871

Purpose

Knockout of human SQLE

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SQLE sgRNA (SQLE Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN-FHA2 R605A-Ypet-34-ECFP-hLaminA

Plasmid

#235123

Purpose

FHA2-mutated FRET biosensor R605A; FRET biosensor for live cell visualization of pSer22 Lamin A

Depositor

Qin Peng

Article

Liu et al Biomater Res. 2024 Oct 22;28:0091. doi:

Insert

FHA2 R605A-Ypet-34-ECFP-hLaminA

Use

Lentiviral

Expression

Mammalian

Mutation

FHA2 R605A

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Actin-16×CBS-GK

Plasmid

#164278

Purpose

Transcribe ACTB-16×CBS mRNA as target for VN-dCasE-VC (VN-dEcCas6-VC-GK) in mammalian cells

Depositor

Chunyu Han

Article

Gao et al Nucleic Acids Res. 2022 May 6;50(8):e46.

Insert

ACTB-16×CBS (ACTB Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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