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pCI-Neo-mdm2H452A/2858

Plasmid

#11497

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Expression

Mammalian

Mutation

H452A

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pCMV_aActTS_indicator

Plasmid

#252385

Purpose

Non-FRET aActinin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aActinin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor_attP-Ef1a-Puro-P2A-mCherry

Plasmid

#247159

Purpose

attP donor plasmid expressing puromycin resistance and mCherry

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LAMP1-Halo

Plasmid

#221655

Purpose

Lysosomal marker LAMP1 tagged C-terminally with Halo tag

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

LAMP1 (Lamp1 Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti NbALFA-mNeonGreen hygro

Plasmid

#193974

Purpose

lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance

Depositor

Raphael Gaudin

Article

Bare et al bioRxiv 2025.10.07.680694

Insert

NbALFA-mNeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SNCA-A53T-mRuby3

Plasmid

#215379

Purpose

Expression of the A53T mutant of alpha-synuclein with a C-terminal mRuby3 tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

SNCA (SNCA Human)

Tags

mRuby3 tag

Expression

Mammalian

Mutation

Ala53Thr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHE111-3xFlag-HSP70

Plasmid

#236055

Purpose

Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv [Preprint]. 2025 Feb 17:2025.02.

Insert

HSP70 (HSPA1A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G_SS-HA-V5-LaccID-CD4 TM_Puro

Plasmid

#234047

Purpose

expresses LaccID on the mammalian cell surface, dox-inducible lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID-CD4TM

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCB094 FoMV-sgLw

Plasmid

#234816

Purpose

To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting SbLw

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCB092 FoMV-sgMgCh

Plasmid

#234815

Purpose

To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting SbMgCh

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMK003

Plasmid

#236570

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISDra2 guides into ISDra2 plant expression vector

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-puro_FlnaABD-TS-16flag

Plasmid

#234868

Purpose

Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

Depositor

Michael Murrell

Article

Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

Insert

FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16

Use

Lentiviral

Tags

GFP, RFP, FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

Plasmid

#234869

Purpose

Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

Depositor

Michael Murrell

Article

Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

Insert

FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24

Use

Lentiviral

Tags

GFP, RFP, FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-FLAG-HA-(N)-CDK12

Plasmid

#231750

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-HA-3C

Expression

Mammalian

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT51

Plasmid

#223423

Purpose

T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-LbCas12a-RRV-2x35s-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstarC

Plasmid

#232737

Purpose

Expresses control sensor NAPstarC in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstarC

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar3

Plasmid

#232740

Purpose

Expresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar3

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDP0437

Plasmid

#221127

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

pHluorin

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-227_Cacnes_mCerulean

Plasmid

#225614

Purpose

Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

mCerulean

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-mito

Plasmid

#229232

Purpose

Expresses GFP localized to mitochondria

Depositor

Suzanne Pfeffer

Article

Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

Insert

EGFP-MAOA (MAOA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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