We narrowed to 168,360 results for: addgene
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Plasmid#230084PurposeCrispr knock out human L2HGDH geneDepositorAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only
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pLentiCRISPR v2-sgL2HGDH-3
Plasmid#230085PurposeCrispr knock out human L2HGDH geneDepositorAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HA-CBS(opt)-FLAG
Plasmid#230088Purposeexpressing human CBSDepositorInsertCBS (CBS Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationMultiple wobble mutationsPromoterCMVAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA LTB4R
Plasmid#226810PurposeTet-inducible lentiviral vector for 2xHA LTB4R expressionDepositorInsertLTB4R (LTB4R Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_858043.1Available SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR153
Plasmid#226808PurposeGateway entry vector for 2xHA GPR153DepositorInsertGPR153 (GPR153 Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_997253.2Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA LTB4R
Plasmid#226806PurposeGateway entry vector for 2xHA LTB4RDepositorInsertLTB4R (LTB4R Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_858043.1Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA GPR137
Plasmid#226813PurposeTet-inducible lentiviral vector for 2xHA GPR137 expressionDepositorInsertGPR137 (GPR137 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_001164351Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-LMNB1
Plasmid#227328PurposeDonor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)DepositorInsertLMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-MAPRE1
Plasmid#227323PurposeDonor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)DepositorInsertMAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-Puro-TP53
Plasmid#227319PurposeDonor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)DepositorInsertTP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-EZR
Plasmid#227294PurposeDonor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)DepositorInsertEZR Homology Arms flanking a mStayGold Tag (EZR Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-H3C2
Plasmid#227334PurposeDonor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)DepositorInsertH3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC030 (∆CBBp ∆MBH ∆SH)
Plasmid#226205PurposeElectroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).DepositorInsertHomology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
UseSynthetic BiologyMutation∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…Available SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC036 (∆pHG1)
Plasmid#226206PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.DepositorInsertHomology arms for the pHG1 megaplasmid replication region
UseSynthetic BiologyMutation∆helD ∆repA ∆repB ∆parABAvailable SinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-Solo-miRFP670nano3-H3C2
Plasmid#227335PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)DepositorInsertH3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-H2BC11-MMEJ
Plasmid#227333PurposeMMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-MAP4
Plasmid#227296PurposeDonor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)DepositorInsertMAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-CEP192
Plasmid#227289PurposeDonor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)DepositorInsertCEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-ARL13B
Plasmid#227277PurposeDonor template for mStayGold insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with sgRNA plasmid pX330-PITCh-ARL13B (Addgene #227276)DepositorInsertARL13B Homology Arms flanking a mStayGold Tag (ARL13B Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-CEP192
Plasmid#227290PurposeDonor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)DepositorInsertCEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only