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pGM117

Plasmid

#220168

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

CISD1 (CISD1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2S1 #2

Plasmid

#202757

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2S1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2S1 #1

Plasmid

#202756

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2S1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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piRFP_SeV_Phosphoprotein

Plasmid

#228807

Purpose

Expression of Sendai virus Phosphoprotein

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

Phosphoprotein

Tags

iRFP670

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-meGFP-DLC1

Plasmid

#192048

Purpose

Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

Depositor

Ionel Popa

Article

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

DLC1 (DLC1 Human)

Expression

Bacterial

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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AiP2040 - pAAV-AiE2615m-SYFP2-WPRE3-BGHpA

Plasmid

#230549

Purpose

AiE2615m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

pCopA

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pADSS-EGFP

Plasmid

#228197

Purpose

expressed human ADSS with an EGFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

Insert

ADSS (ADSS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a_SMOX

Plasmid

#186345

Purpose

C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Tags

His tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLPE1

Plasmid

#209981

Purpose

Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_ampR_B0017)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPE2

Plasmid

#209982

Purpose

Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (ampR_B0017)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPE3

Plasmid

#209985

Purpose

Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_cat)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPR3

Plasmid

#209986

Purpose

Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (ermBL)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-FKBP-NMD3

Plasmid

#221296

Purpose

Expression of NMD3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

NMD3 (pLxIS) (Nmd3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TMPRSS3

Plasmid

#221298

Purpose

Expression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TMPRSS3 (pLxIS) (Tmprss3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-mApple-he1.1:CFP

Plasmid

#184814

Purpose

Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-mApple-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-mApple

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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SH3PXD2A-PX (1-126)

Plasmid

#119115

Purpose

Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SH3PXD2A-PX (1-126) (SH3PXD2A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT_WPRE

Plasmid

#216414

Purpose

Encodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 dUBA (1-389)

Plasmid

#210426

Purpose

Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL dUBA (1-389) (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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