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pML104-HIS3-3

Plasmid

#232882

Purpose

Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-YFR054C

Plasmid

#232900

Purpose

Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

YFR054C gRNA (YFR054C Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ZIM17

Plasmid

#232901

Purpose

Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ZIM17 gRNA (ZIM17 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SRP14

Plasmid

#232896

Purpose

Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SRP14 gRNA (SRP14 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-TLG1

Plasmid

#232897

Purpose

Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

TLG1 gRNA (TLG1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-VHT1

Plasmid

#232898

Purpose

Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VHT1 gRNA (VHT1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-2

Plasmid

#232881

Purpose

Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ADE13

Plasmid

#232887

Purpose

Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ADE13 gRNA (ADE13 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-LAS17

Plasmid

#232892

Purpose

Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LAS17 gRNA (LAS17 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#232883

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRS313-rad53-5004

Plasmid

#232902

Purpose

Plasmid carrying the rad53-5004 allele with its native promoter and terminator.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

RAD53 (RAD53 Budding Yeast)

Expression

Yeast

Mutation

T600C, A680G, A1094T, C1615T, C2228A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-INO80

Plasmid

#232890

Purpose

Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

INO80 gRNA (INO80 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-zdgat2FLAG-SV40

Plasmid

#232141

Purpose

Expresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Zebrafish expression

Tags

3xFLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pXMLC2-DEST (JDW 7)

Plasmid

#229833

Purpose

A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway destination vector

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-BFP (JDW 491)

Plasmid

#229834

Purpose

A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-StayGoldc4-Giantin (JDW 1355)

Plasmid

#229844

Purpose

A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

n2StayGoldc4

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zdgat2-CDS-FLAG

Plasmid

#232142

Purpose

Gateway middle entry vector containing zebrafish dgat2 with a C-terminal 3xFLAG tag

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Gateway cloning middle entry

Tags

3xFLAG

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ HA BEX3

Plasmid

#231004

Purpose

Transient overexpression of human BEX3 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX3 (BEX3 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2 3xFLAG FEM1A

Plasmid

#231001

Purpose

Transient overexpression of mouse FEM1A in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

FEM1A (Fem1a Mouse)

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCJ1023

Plasmid

#228763

Purpose

Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

human U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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